Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197618
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleElectrocatalytic Determination Of Reduced Glutathione In Human Erythrocytes.pt_BR
dc.contributor.authorde Cássia Silva Luz, Ritapt_BR
dc.contributor.authorDamos, Flavio Santospt_BR
dc.contributor.authorGandra, Paulo Guimarãespt_BR
dc.contributor.authorde Macedo, Denise Vazpt_BR
dc.contributor.authorTanaka, Auro Atsushipt_BR
dc.contributor.authorKubota, Lauro Tatsuopt_BR
unicamp.authorRita de Cássia Silva Luz, Institute of Chemistry, UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil.pt_BR
unicamp.author.externalFlavio Santos Damos,pt
unicamp.author.externalPaulo Guimarães Gandra,pt
unicamp.author.externalDenise Vaz de Macedo,pt
unicamp.author.externalAuro Atsushi Tanaka,pt
unicamp.author.externalLauro Tatsuo Kubota,pt
dc.subjectAdultpt_BR
dc.subjectBiosensing Techniquespt_BR
dc.subjectBlood Chemical Analysispt_BR
dc.subjectElectrochemistrypt_BR
dc.subjectErythrocytespt_BR
dc.subjectFemalept_BR
dc.subjectGlutathionept_BR
dc.subjectHumanspt_BR
dc.subjectMalept_BR
dc.subjectMiddle Agedpt_BR
dc.subjectReproducibility Of Resultspt_BR
dc.subjectSensitivity And Specificitypt_BR
dc.description.abstractThe determination of reduced glutathione (GSH) in human erythrocytes using a simple, fast and sensitive method employing a glassy carbon electrode modified with cobalt tetrasulfonated phthalocyanine (CoTSPc) immobilized in poly(L: -lysine) (PLL) film was investigated. This modified electrode showed very efficient electrocatalytic activity for anodic oxidation of GSH, decreasing substantially the anodic overpotentials for 0.2 V versus Ag/AgCl. The modified electrode presented better performance in 0.1 mol l(-1) piperazine-N,N'-bis(2-ethanesulfonic acid) buffer at pH 7.4. The other experimental parameters, such as the concentration of CoTSPc and PLL in the membrane preparation, pH, type of buffer solution and applied potential, were optimized. Under optimized operational conditions, a linear response from 50 to 2,160 nmol l(-1) was obtained with a high sensitivity of 1.5 nA l nmol(-1) cm(-2). The detection limit for GSH determination was 15 nmol l(-1). The proposed sensor presented good repeatability, evaluated in terms of the relative standard deviation (1.5%) for n = 10. The modified electrode was applied for determination of GSH in erythrocyte samples and the results were in agreement with those obtained by a comparative method described in the literature The average recovery for these fortified samples was 100 +/- 1)%. Applying a paired Student's-t test to compare these methods, we could observe that, at the 95% confidence level, there was no statistical difference between the reference and the proposed methods.en
dc.relation.ispartofAnalytical And Bioanalytical Chemistrypt_BR
dc.relation.ispartofabbreviationAnal Bioanal Chempt_BR
dc.date.issued2007-Marpt_BR
dc.identifier.citationAnalytical And Bioanalytical Chemistry. v. 387, n. 5, p. 1891-7, 2007-Mar.pt_BR
dc.language.isoengpt_BR
dc.description.volume387pt_BR
dc.description.firstpage1891-7pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1618-2642pt_BR
dc.identifier.doi10.1007/s00216-006-1053-6pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/17200855pt_BR
dc.date.available2015-11-27T13:10:47Z-
dc.date.accessioned2015-11-27T13:10:47Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:10:47Z (GMT). No. of bitstreams: 1 pmed_17200855.pdf: 182186 bytes, checksum: 76d0eea95b1567954989912b19225121 (MD5) Previous issue date: 2007en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197618-
dc.identifier.idPubmed17200855pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_17200855.pdf177.92 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.