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Type: Artigo de periódico
Title: Clonazepam Quantification In Human Plasma By High-performance Liquid Chromatography Coupled With Electrospray Tandem Mass Spectrometry In A Bioequivalence Study.
Author: Cavedal, Luiz E
Mendes, Fabiana D
Domingues, Claudia C
Patni, Anil K
Monif, Tausif
Reyar, Simrit
Pereira, Alberto Dos S
Mendes, Gustavo D
De Nucci, Gilberto
Abstract: A rapid, sensitive and specific method for quantifying clonazepam in human plasma using diazepam as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by liquid-liquid extraction using a hexane/diethylether (20 : 80, v/v) solution. The extracts were analysed by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS-MS). Chromatography was performed on a Jones Genesis C8 4 microm analytical column (100 x 2.1 mm i.d.). The method had a chromatographic run time of 3.0 min and a linear calibration curve over the range 0.5-50 ng/ml (r2 > 0.9965). The limit of quantification was 0.5 ng/ml. This HPLC/MS/MS procedure was used to assess the bioequivalence of two clonazepam 2 mg tablet formulations (clonazepam test formulation from Ranbaxy Laboratories Ltd and Rivotril from Roche Laboratórios Ltda as standard reference formulation).
Subject: Adolescent
Chromatography, High Pressure Liquid
Cross-over Studies
Middle Aged
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Therapeutic Equivalency
Citation: Journal Of Mass Spectrometry : Jms. v. 42, n. 1, p. 81-8, 2007-Jan.
Rights: fechado
Identifier DOI: 10.1002/jms.1141
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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