Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197451
Type: Artigo de periódico
Title: Induction Of Apoptosis In Caco-2 And Ht-29 Human Intestinal Epithelial Cells By Enterohemolysin Produced By Classic Enteropathogenic Escherichia Coli.
Author: Figueiredo, P M S
Furumura, M T
Aidar-Ugrinovich, L
Pestana de Castro, A F
Pereira, F G
Metze, I L
Yano, T
Abstract: Detect the cytotoxic effects of the Enterohemolysin from enteropathogenic Escherichia coli C3888 (O 26: H-) on Caco 2 and HT-29-human epithelial intestinal cells. The Caco 2 and HT-29 cells, which were treated with Enterohemolysin (EHly) within 10-15 min, became round, lost attachment to substrate, showed extensive surface blebbing, nucleus shrank, and the chromatin became more compact. After 10 min of exposure to the EHly, the cells showed lactate dehydrogenase (LDH) leakage and reduction of mitochondrial activity. The cells showed disorganization of the actin fibers at 15 min. The death of these human epithelial intestinal cells by apoptosis was confirmed by annexin V. Enterohemolysin induced apoptosis on human epithelial intestinal cells. The finding of EHly cytotoxic activity suggests the involvement of this hemolysin in the (Enteropathogenic Escherichia coli) EPEC infection mechanism and may facilitate the understanding of the diarrhea caused by EPEC.
Subject: Alkaline Phosphatase
Apoptosis
Caco-2 Cells
Cell Survival
Diarrhea
Escherichia Coli
Escherichia Coli Infections
Escherichia Coli Proteins
Ht29 Cells
Hemolysin Proteins
Humans
Intestinal Mucosa
L-lactate Dehydrogenase
Rights: fechado
Identifier DOI: 10.1111/j.1472-765X.2007.02154.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/17868319
Date Issue: 2007
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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