Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197446
Type: Artigo de periódico
Title: Dynamics Of The Epithelium During Canalization Of The Rat Ventral Prostate.
Author: Bruni-Cardoso, Alexandre
Carvalho, Hernandes F
Abstract: Outgrowth and branching of solid cords are the initial events in postnatal prostatic morphogenesis. These processes involve cell proliferation and their projection into the stroma and precede epithelial canalization. The purpose of the present study was to examine the dynamics of the prostate epithelium during canalization of the rat ventral prostate in the first week of postnatal development using histological, stereological, and ultrastructural analyses. The terminal deoxynucleotidyltransferase [TdT]-mediated deoxy-UTP nick end labeling assay was used to investigate the occurrence of DNA fragmentation. Our results demonstrate that canalization of the prostate epithelium starts as early as on day 1 (24 hr after birth) and progresses thereafter. By the end of the first week (day 6), luminal volume density reached approximately 3% (P < 0.05) of the organ. Canalization was the result of epithelial cell differentiation and apoptosis. The former involved organization of the epithelial cells into a single layer sitting on the basement membrane, polarization, enlargement of secretory organelles and accumulation of secretory vesicles, microvilli formation, and establishment of the adult pattern of cell junctions. The latter was observed to occur mostly to epithelial cells not in contact with the basement membrane. Structures of variable electron density were observed in the developing lumen. In conclusion, different phenomena seem to be involved in the canalization of the rat ventral prostate. However, it was evident from the present results that complex epithelial cell fate decisions take place during this process.
Subject: Animals
Apoptosis
Biometry
Dna Fragmentation
Epithelial Cells
Epithelium
In Situ Nick-end Labeling
Indoles
Male
Prostate
Rats
Rats, Wistar
Rights: fechado
Identifier DOI: 10.1002/ar.20591
Address: http://www.ncbi.nlm.nih.gov/pubmed/17847055
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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