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Type: Artigo de periódico
Title: An Anticarsia Gemmatalis Multiple Nucleopolyhedrovirus Mutant, Vapag, Induces Hemocytes Apoptosis In Vivo And Displays Reduced Infectivity In Larvae Of Anticarsia Gemmatalis (hübner) (lepidoptera: Noctuidae).
Author: da Silveira, Eni Braga
Cordeiro, Bruno Arrivabene
Ribeiro, Bergmann Morais
de Castro, Maria Elita Batista
Soares, Elisa Filgueiras
Báo, Sônia Nair
Abstract: An Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) mutant, vApAg, induces apoptosis in a cell culture derived from Anticarsia gemmatalis (UFL-AG-286), reducing viral progeny. We have investigated apoptosis induction in vivo by vApAg in A. gemmatalis larvae and its correlation to infectivity reduction. LC(50), LD(50), LT(50) and the mean time to death of larvae were determined for vApAg and AgMNPV. Apoptosis was accessed for hemocytes of infected larvae using light and transmission electron microscopy. All types of hemocytes can be infected by vApAg. After 12h post-infection (h p.i.), typical cellular modifications associated to nucleopolyhedrovirus infection were observed. Apoptosis becomes evident after 24h p.i., and massive after 72h p.i. Necrosis of infected cells was also observed. Despite cell death, hemocytes produced budded viruses and polyhedra. Pl and gh1-type hemocytes presented phagocytic activity. Agarose gel electrophoresis revealed fragmentation of hemocytes DNA at late times post-infection. The LC(50) and LD(50) were between five- and six-fold higher for vApAg. The LT(50) and the mean time to death were higher for vApAg in a same treatment or for a similar mortality induced by AgMNPV. These results show correlation of apoptosis and the reduced infectivity of vApAg in A. gemmatalis larvae.
Subject: Animals
Cells, Cultured
Dna Fragmentation
Lethal Dose 50
Microscopy, Electron, Transmission
Survival Analysis
Time Factors
Rights: fechado
Identifier DOI: 10.1016/j.virusres.2007.06.010
Date Issue: 2007
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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