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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleThe Use Of Asb-14 In Combination With Chaps Is The Best For Solubilization Of Human Brain Proteins For Two-dimensional Gel Electrophoresis.pt_BR
dc.contributor.authorMartins-de-Souza, Danielpt_BR
dc.contributor.authorMartins, Danielpt_BR
dc.contributor.authorMenezes de Oliveira, Brunopt_BR
dc.contributor.authordos Santos Farias, Alessandropt_BR
dc.contributor.authorHoriuchi, Ricardo Shiniti Okapt_BR
dc.contributor.authorCrepaldi Domingues, Cleytonpt_BR
dc.contributor.authorde Paula, Eneidapt_BR
dc.contributor.authorMarangoni, Sérgiopt_BR
dc.contributor.authorGattaz, Wagner Faridpt_BR
dc.contributor.authorDias-Neto, Emmanuelpt_BR
dc.contributor.authorCamillo Novello, Josépt_BR
unicamp.authorDaniel Martins-de-Souza, Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil. daniel@proteome.ibi.unicamp.brpt_BR
unicamp.author.externalDaniel Martins,pt
unicamp.author.externalBruno Menezes de Oliveira,pt
unicamp.author.externalAlessandro dos Santos Farias,pt
unicamp.author.externalRicardo Shiniti Oka Horiuchi,pt
unicamp.author.externalCleyton Crepaldi Domingues,pt
unicamp.author.externalEneida de Paula,pt
unicamp.author.externalSérgio Marangoni,pt
unicamp.author.externalWagner Farid Gattaz,pt
unicamp.author.externalEmmanuel Dias-Neto,pt
unicamp.author.externalJosé Camillo Novello,pt
dc.subjectBetainept_BR
dc.subjectBrain Chemistrypt_BR
dc.subjectCholic Acidspt_BR
dc.subjectDetergentspt_BR
dc.subjectElectrophoresis, Gel, Two-dimensionalpt_BR
dc.subjectHumanspt_BR
dc.subjectProteinspt_BR
dc.subjectSolubilitypt_BR
dc.description.abstractProtein extraction is the most important step to reveal a proteome by Two-Dimensional Gel Electrophoresis. Usually, the urea/thiourea based standard protein extraction buffer (SB) is combined with detergents with the aim of achieving better resolution and solubilization of different classes of proteins. In order to produce better gels and achieve the greatest spot resolution of Human Brain Proteins, comparisons using 2-DE of extracted proteins from Human Brain Frontal Cortex with SB constituents (7M Urea, 2M Thiourea and 100mM DTT) were made, using different detergent compositions in the buffer. SB preparations in combination with CHAPS and ASB-14 as well as with ASB-16 (reported for the first time in 2-DE experiments) have been tested. Our results confirm that the most efficient solubilizing solution for 2-DE analysis of cytosolic and membrane Human Brain Proteins is SB combined with 4% CHAPS and 2% ASB-14.en
dc.relation.ispartofBriefings In Functional Genomics & Proteomicspt_BR
dc.relation.ispartofabbreviationBrief Funct Genomic Proteomicpt_BR
dc.date.issued2007-Marpt_BR
dc.identifier.citationBriefings In Functional Genomics & Proteomics. v. 6, n. 1, p. 70-5, 2007-Mar.pt_BR
dc.language.isoengpt_BR
dc.description.volume6pt_BR
dc.description.firstpage70-5pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1473-9550pt_BR
dc.identifier.doi10.1093/bfgp/elm009pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/17556486pt_BR
dc.date.available2015-11-27T13:10:10Z-
dc.date.accessioned2015-11-27T13:10:10Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:10:10Z (GMT). No. of bitstreams: 1 pmed_17556486.pdf: 286013 bytes, checksum: 25ace51e6b822b8d4d6e7b23defce7d8 (MD5) Previous issue date: 2007en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197324-
dc.identifier.idPubmed17556486pt_BR
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