Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197281
Type: Artigo de periódico
Title: Acetylcholine Receptor Organization At The Dystrophic Extraocular Muscle Neuromuscular Junction.
Author: Marques, Maria Julia
Pertille, Adriana
Carvalho, Candida Luiza T
Santo Neto, Humberto
Abstract: Spared extraocular muscles of dystrophic mice are not subjected to regeneration process and can be used to verify whether the lack of dystrophin per se could cause changes in acetylcholine receptor (AChR) distribution. In the present study, rectus and oblique (spared) and retractor bulbi (nonspared) muscles were dissected from adult control (C57Bl/10) and mdx mice. AChRs and nerve terminals were labeled with rhodamine-alpha-bungarotoxin and anti-NF200-IgG-FITC, respectively, and visualized by confocal microscopy. Rectus and oblique muscles presented 0.5% central nucleation, while retractor bulbi had central nucleation in 45% of muscle fibers. In mdx rectus, AChRs were distributed in branches in 99% of the junctions examined (n = 200), similar to that observed for controls. Nerve terminals covered the AChR branches in 100% of the junctions examined. In control retractor bulbi, AChRs were distributed in regular branches. In mdx retractor bulbi, multiple fragmented islands of receptors were seen in 56% of the endplates examined (n = 200). These results suggest that the lack of dystrophin per se does not influence the distribution of acetylcholine receptors at the neuromuscular junction of spared extraocular muscles.
Subject: Animals
Bungarotoxins
Disease Models, Animal
Dystrophin
Fluorescent Antibody Technique
Fluorescent Dyes
Male
Mice
Mice, Inbred C57bl
Mice, Inbred Mdx
Microscopy, Confocal
Muscle, Skeletal
Muscular Dystrophy, Animal
Muscular Dystrophy, Duchenne
Neurofilament Proteins
Neuromuscular Junction
Presynaptic Terminals
Receptors, Cholinergic
Regeneration
Rhodamines
Citation: Anatomical Record (hoboken, N.j. : 2007). v. 290, n. 7, p. 846-54, 2007-Jul.
Rights: fechado
Identifier DOI: 10.1002/ar.20525
Address: http://www.ncbi.nlm.nih.gov/pubmed/17492672
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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