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Type: Artigo de periódico
Title: Cloud Point Extraction Applied To Casein Proteins Of Cow Milk And Their Identification By Mass Spectrometry.
Author: Lopes, Aline Soriano
Garcia, Jerusa Simone
Catharino, Rodrigo Ramos
Santos, Leonardo Silva
Eberlin, Marcos Nogueira
Arruda, Marco Aurélio Zezzi
Abstract: This work describes the optimization of a cloud point extraction (CPE) method for casein proteins from cow milk samples. To promote phase separation, polyoxyethylene(8) isooctylphenyl ether (Triton X-114) and sodium chloride (NaCl) were used as nonionic surfactant and electrolyte, respectively. Using multivariate studies, four major CPE variables were evaluated: Triton X-114 concentration, sample volume, NaCl concentration, and pH. The results show that surfactant concentration and sample volume were the main variable affecting the CPE process, with the following optimized parameters: 1% (w/v) Triton X-114 concentration, 50 microL of sample volume, 6% (w/v) NaCl concentration and extractions carried out at pH 7.0. At these conditions, 923+/-66 and 67+/-2 microg mL(-1) of total protein were found in the surfactant-rich and surfactant-poor phases, respectively. Finally, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was then used to evaluate those target proteins (alpha(s1)-casein, alpha(s2)-casein and beta-casein) separation as well as to check the efficiency of the extraction procedure, making a fingerprint of those target proteins possible.
Subject: Animals
Polyethylene Glycols
Sodium Chloride
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Citation: Analytica Chimica Acta. v. 590, n. 2, p. 166-72, 2007-May.
Rights: fechado
Identifier DOI: 10.1016/j.aca.2007.03.043
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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