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Type: Artigo de periódico
Title: Purification Of The Seven Tetranortriterpenoids In Neem (azadirachta Indica) Seed By Counter-current Chromatography Sequentially Followed By Isocratic Preparative Reversed-phase High-performance Liquid Chromatography.
Author: Silva, Júlio César T
Jham, Gulab N
Oliveira, Rosângela D'arc L
Brown, Leslie
Abstract: Counter-current chromatography (CCC) sequentially followed by isocratic preparative reversed-phase high-performance liquid chromatography was used to isolate the seven bio-actives (azadirachtin A, azadirachtin B, azadirachtin H, desacetylnimbin, desacetylsalannin, nimbin and salannin) from the seed concentrate (NSC) of the neem tree (Azadirachta indica A. Juss). Reproducible, narrow polarity range, high purity fractions were obtained from repeated injections of the NSC (700 mg loadings/injection), on to a relatively small volume CCC coil (116 mL). The CCC biphasic solvent system chosen was hexane:butanol:methanol:water (1:0.9:1:0.9, v/v). A mass balance of injected material showed that 95+% were recovered.
Subject: Azadirachta
Chromatography, High Pressure Liquid
Countercurrent Distribution
Molecular Structure
Reproducibility Of Results
Citation: Journal Of Chromatography. A. v. 1151, n. 1-2, p. 203-10, 2007-Jun.
Rights: fechado
Identifier DOI: 10.1016/j.chroma.2007.03.086
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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