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Type: Artigo de periódico
Title: Determination Of Pesticides And Metabolites In Wine By High Performance Liquid Chromatography And Second-order Calibration Methods.
Author: Braga, Jez Willian B
Bottoli, Carla B G
Jardim, Isabel C S F
Goicoechea, Héctor C
Olivieri, Alejandro C
Poppi, Ronei J
Abstract: The models parallel factor analysis (PARAFAC) and the recently introduced bilinear least squares (BLLS) were applied to develop second-order calibration methods to high performance liquid chromatography with diode array detection (HPLC-DAD) data, where overlap of interferences with the compounds of interest was observed, making the determination and resolution of the analytes possible. In this work, the simultaneous determination of five pesticides and two metabolites in wine samples by HPLC-DAD was performed, using the second-order advantage. The results of two chromatographic methods were compared, involving either isocratic or gradient elution. An appropriate preprocessing method was necessary to correct the effects of time shifts, baseline variations and background. BLLS presented results that were of the same quality as PARAFAC in five cases, but in two other situations only PARAFAC enabled analyte quantitation. Relative errors of prediction lower than 10% for all compounds were obtained, indicating that the methodology employing HLPC-DAD and second-order calibration can handle complex analytical systems.
Subject: Algorithms
Chromatography, High Pressure Liquid
Least-squares Analysis
Models, Statistical
Pesticide Residues
Reproducibility Of Results
Solid Phase Extraction
Citation: Journal Of Chromatography. A. v. 1148, n. 2, p. 200-10, 2007-May.
Rights: fechado
Identifier DOI: 10.1016/j.chroma.2007.03.018
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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