Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197217
Type: Artigo de periódico
Title: Protective Effect Of Prior Physical Conditioning On Relaxing Response Of Corpus Cavernosum From Rats Made Hypertensive By Nitric Oxide Inhibition.
Author: Claudino, M A
Priviero, F B M
Camargo, E A
Teixeira, C E
De Nucci, G
Antunes, E
Zanesco, A
Abstract: The aim of this work was to evaluate the influence of run training on the responsiveness of corpus cavernosum (CC) from rats made hypertensive by treatment with nitric oxide (NO) synthesis inhibitor. Wistar rats were divided into sedentary control (C-SD), exercise training (C-TR), N(omega)-nitro-L-arginine methyl ester (L-NAME) sedentary (LN-SD) and L-NAME trained (LN-TR) groups. The run training program consisted in 8 weeks in a treadmill, 5 days/week, each session lasted 60 min. L-NAME treatment (2 and 10 mg/rat/day) started after 4 weeks of prior physical conditioning and lasted 4 weeks. Concentration-response curves were obtained for acetylcholine (ACh), sodium nitroprusside (SNP), sildenafil and BAY 41-2272. The effect of electrical field stimulation (EFS) on the relaxations responses of CC was evaluated. Run training prevented the arterial hypertension induced by L-NAME treatment (LN-SD: 135+/-2 and 141+/-2 mm Hg for both doses of L-NAME) compared to LN-SD groups (154+/-1 and 175+/-2 mm Hg, for 2 and 10 mg of L-NAME, respectively). Run training produced an increase in the maximal responses (E(max)) of CC for ACh (C-SD: 47+/-3; C-TR: 52+/-1; and LN-TR: 53+/-3%) and SNP (C-SD: 89+/-1; C-TR: 98+/-1; and LN-TR: 95+/-1%). Both potency and E(max) for ACh were reduced in a dose of 10 mg of L-NAME, and run training restored the reduction of E(max) for ACh. No changes were found for BAY 41-2271 and sildenafil. Relaxing responses to EFS was reduced by L-NAME treatment that was restored by prior physical conditioning. In conclusion, our study shows a beneficial effect of prior physical conditioning on the impaired CC relaxing responses in rats made hypertensive by chronic NO blockade.
Subject: Acetylcholine
Animals
Blood Pressure
Body Weight
Dose-response Relationship, Drug
Electric Stimulation
Enzyme Inhibitors
Hypertension
In Vitro Techniques
Male
Muscle Relaxation
Ng-nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Nitroprusside
Penis
Physical Conditioning, Animal
Piperazines
Purines
Pyrazoles
Pyridines
Rats
Rats, Wistar
Sulfones
Rights: fechado
Identifier DOI: 10.1038/sj.ijir.3901511
Address: http://www.ncbi.nlm.nih.gov/pubmed/16915305
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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