Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197193
Type: Artigo de periódico
Title: Role Of 17 Beta-estradiol On Type Iv Collagen Fibers Volumetric Density In The Basement Membrane Of Bladder Wall.
Author: de Fraga, Rogerio
Dambros, Miriam
Miyaoka, Ricardo
Riccetto, Cássio Luís Zanettini
Palma, Paulo César Rodrigues
Abstract: The authors quantified the type IV collagen fibers volumetric density in the basement membrane of bladder wall of ovariectomized rats with and without estradiol replacement. This study was conducted on 40 Wistar rats (3 months old) randomly divided in 4 groups: group 1, remained intact (control); group 2, submitted to bilateral oophorectomy and daily replacement 4 weeks later of 17 beta-estradiol for 12 weeks; group 3, sham operated and daily replacement 4 weeks later of sesame oil for 12 weeks; and group 4, submitted to bilateral oophorectomy and killed after 12 weeks. It was used in immunohistochemistry evaluation using type IV collagen polyclonal antibody to stain the fibers on paraffin rat bladder sections. The M-42 stereological grid system was used to analyze the fibers. Ovariectomy had an increase effect on the volumetric density of the type IV collagen fibers in the basement membrane of rat bladder wall. Estradiol replacement in castrated animals demonstrated a significative difference in the stereological parameters when compared to the castrated group without hormonal replacement. Surgical castration performed on rats induced an increasing volumetric density of type IV collagen fibers in the basement membrane of rats bladder wall and the estradiol treatment had a significant effect in keeping a low volumetric density of type IV collagen fibers in the basement membrane of rats bladder wall.
Subject: Animals
Basement Membrane
Collagen Type Iv
Estradiol
Female
Immunohistochemistry
Ovariectomy
Rats
Rats, Wistar
Urinary Bladder
Rights: fechado
Identifier DOI: 10.1007/s00192-006-0294-4
Address: http://www.ncbi.nlm.nih.gov/pubmed/17347794
Date Issue: 2007
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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