Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197154
Type: Artigo de periódico
Title: Tetrahydroxyquinone Induces Apoptosis Of Leukemia Cells Through Diminished Survival Signaling.
Author: Martins Cavagis, Alexandre D
Ferreira, Carmen Veríssima
Versteeg, Henri H
Assis, Cristiane Fernandes
Bos, Carina L
Bleuming, Sylvia A
Diks, Sander H
Aoyama, Hiroshi
Peppelenbosch, Maikel P
Abstract: Tetrahydroxyquinone is a molecule best known as a primitive anticataract drug but is also a highly redox active molecule that can take part in a redox cycle with semiquinone radicals, leading to the formation of reactive oxygen species (ROS). Its potential as an anticancer drug has not been investigated. The effects of tetrahydroxyquinone on HL60 leukemia cells are investigated using fluorescein-activated cell sorting-dependent detection of phosphatidylserine exposure combined with 7-amino-actinomycin D exclusion, via Western blotting using phosphospecific antibodies, and by transfection of constitutively active protein kinase B. We observe that in HL60 leukemia cells tetrahydroxyquinone causes ROS production followed by apoptosis through the mitochondrial pathway, whereas cellular physiology of normal human blood leukocytes was not affected by tetrahydroxyquinone. The antileukemic effect of tetrahydroxyquinone is accompanied by reduced activity of various antiapoptotic survival molecules including the protein kinase B pathway. Importantly, transfection of protein kinase B into HL60 cells and thus artificially increasing protein kinase B activity inhibits tetrahydroxyquinone-dependent cytotoxicity. Tetrahydroxyquinone provokes cytotoxic effects on leukemia cells by reduced protein kinase B-dependent survival signaling followed by apoptosis through the mitochondrial pathway. Thus, tetrahydroxyquinone may be representative of a novel class of chemotherapeutic drugs, inducing apoptosis in cancer cells through diminished survival signaling possibly as a consequence of ROS generation.
Subject: Apoptosis
Cell Survival
Hl-60 Cells
Humans
Hydroquinones
Jnk Mitogen-activated Protein Kinases
Leukemia
Leukocytes, Mononuclear
Mitochondria
Phosphoprotein Phosphatases
Proto-oncogene Proteins C-akt
Quinones
Reactive Oxygen Species
Signal Transduction
Tumor Cells, Cultured
Citation: Experimental Hematology. v. 34, n. 2, p. 188-96, 2006-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.exphem.2005.11.001
Address: http://www.ncbi.nlm.nih.gov/pubmed/16459187
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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