Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197152
Type: Artigo de periódico
Title: Effects Of Veratrine On Skeletal Muscle Mitochondria: Ultrastructural, Cytochemical, And Morphometrical Studies.
Author: Freitas, Erika Maria Silva
Fagian, Márcia M
da Cruz-Höfling, Maria Alice
Abstract: The alkaloid veratrine is a lipid-soluble neurotoxin, which target voltage-gated Na+ channels for their primary action. Recently, we showed that this alkaloid may cause myonecrosis and evidences suggest mitochondria as one of its cell targets. Herein, we investigate the effects caused by variable concentration of veratrine (250 and 550 microg/mL) on mitochondrial oxygen consumption, respiratory chain enzymes activities, and ultrastructure, combining electron microscopy with cytochemical and biochemical approaches. The results showed different sort of ultrastructural changes, both in isolated and intramuscular mitochondria. Veratrine decreased mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH-d), succinic dehydrogenase (SDH), and cytochrome oxidase (COX) activities, significantly and dose-dependently inhibited the state 3 respiration rate, respiratory control ratio (RCR), and ADP/O on isolated rat skeletal muscle mitochondria, whereas state 4 was unaffected. A tendency of increase in mitochondria diameter was seen with 250 microg/mL veratrine. We conclude that the alkaloid would probably act on mitochondrial membrane phospholipid configuration, which would explain the changes observed.
Subject: Animals
Electron Transport
Electron Transport Complex Iv
Histocytochemistry
Mice
Microscopy, Electron, Transmission
Mitochondria, Muscle
Mitochondrial Membranes
Models, Animal
Muscle, Skeletal
Nadh Dehydrogenase
Oxygen Consumption
Rats
Succinate Dehydrogenase
Veratrine
Rights: fechado
Identifier DOI: 10.1002/jemt.20280
Address: http://www.ncbi.nlm.nih.gov/pubmed/16456834
Date Issue: 2006
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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