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Type: Artigo de periódico
Title: Cytokine Gene Expression In Walker 256: A Comparison Of Variants A (aggressive) And Ar (regressive).
Author: De Almeida Salles Perroud, Ana Paula
Ashimine, Rika
De Castro, Glaucia Monteiro
Guimarães, Fernando
Vieira, Karla Priscila
Aparecida Vilella, Conceição
Samico Cavalcanti, Tereza Cristina
De Lima Zollner, Ricardo
Abstract: Two variants of this Walker 256 tumor have been previously reported as Walker 256 A and variant AR. The variant A has more aggressive property than variant AR and can induce systemic effects such as anorexia, sodium and water retention, followed by weight loss and death. The mechanisms involved in enhancing tumor regression and progression in this model are still incompletely understood. In the present study, serum and spleen mononuclear cells and tumor cells from animals inoculated with variants A and AR, were isolated to investigate the TGF-beta, IL-12, IFN-gamma and TNF-alpha and relationship with anemia, weight of animals, weight of spleen, volume of tumor and osmotic fragility compared with controls inoculated with Ringer Lactate. Results demonstrate that the group inoculated with variant A, compared to variant AR, shows high levels of TGF-beta gene expression in both tumor tissue and spleen cells, no expression of IFN-gamma and a progressive and higher levels of IL-12 in tumor tissue without inflammatory infiltrate visualized by optical microscopy. These results suggest that the aggressively of variant A is relate to cytokine modulation, facilitating the growth and escape of tumor cells. Furthermore, IL-12 seems to be constitutively expressed in both tumor lineage A and AR.
Subject: Anemia
Body Weight
Carcinoma 256, Walker
Enzyme-linked Immunosorbent Assay
Gene Expression
Organ Size
Osmotic Fragility
Rna, Messenger
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Transforming Growth Factor Beta
Tumor Necrosis Factor-alpha
Citation: Cytokine. v. 36, n. 3-4, p. 123-33, 2006-Nov.
Rights: fechado
Identifier DOI: 10.1016/j.cyto.2006.11.004
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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