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Type: Artigo de periódico
Title: Phylogenetic Relationships Of Brazilian Bovine Respiratory Syncytial Virus Isolates And Molecular Homology Modeling Of Attachment Glycoprotein.
Author: Spilki, Fernando Rosado
Almeida, Renata Servan
Domingues, Helena Gallichio
D'Arce, Regina Celia Freitas
Ferreira, Helena Lage
Campalans, Jaqueline
Costa, Sandra Cecília Botelho
Arns, Clarice Weis
Abstract: Bovine respiratory syncytial virus (BRSV) causes lower respiratory tract disease in young cattle. Recently, it was possible to determine the sequence of the G protein gene, which plays a role in the attachment of BRSV particles to the cells, from three distinct Brazilian isolates. The phylogenetic analysis conducted here using those sequences compared to other worldwide distributed isolates of BRSV allow us to allocate Brazilian strains within the subgroup B, which was no longer found in the world since the 1970s. One of the Brazilian strains has a major mutation between amino acid residues 173 and 178, within the central hydrophobic conserved region, exactly on the site of two of the four cysteine-noose forming cysteine residues. Homology modeling with the previously determined NMR structure of this protein domain was made to check whether these mutations altered the three-dimensional conformation of this immunodominant region. Possible consequences on the biological effects induced by such mutation on the G protein are discussed.
Subject: Amino Acid Sequence
Computational Biology
Models, Molecular
Molecular Sequence Data
Protein Structure, Tertiary
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Bovine
Sequence Homology, Amino Acid
Viral Envelope Proteins
Citation: Virus Research. v. 116, n. 1-2, p. 30-7, 2006-Mar.
Rights: fechado
Identifier DOI: 10.1016/j.virusres.2005.08.010
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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