Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197005
Type: Artigo de periódico
Title: Increased Expression Of Snare Proteins And Synaptotagmin Iv In Islets From Pregnant Rats And In Vitro Prolactin-treated Neonatal Islets.
Author: Cunha, Daniel A
Amaral, Maria E C
Carvalho, Carolina P F
Collares-Buzato, Carla B
Carneiro, Everardo M
Boschero, Antonio C
Abstract: During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic beta-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and confocal microscopy. Transcription and expression of all SNARE genes and proteins were increased in islets from pregnant and PRL-treated neonatal rats when compared with controls. The only exception was VAMP-2 production in islets from pregnant rats. Increased mRNA and protein expression of synaptotagmin IV, but not the isoform I, also was observed in islets from pregnant and PRL-treated rats. This effect was not inhibited by wortmannin or PD098059, inhibitors of the PI3-kinase and MAPK pathways, respectively. As revealed by confocal laser microscopy, both syntaxin 1A and synaptotagmin IV were immunolocated in islet cells, including the insulin-containing cells. These results indicate that PRL modulates the final steps of insulin secretion by increasing the expression of proteins involved in membrane fusion.
Subject: Animals
Animals, Newborn
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Female
Gene Expression Regulation, Developmental
Immunoblotting
Immunochemistry
Insulin
Islets Of Langerhans
Microscopy, Confocal
Pregnancy
Prolactin
Rna, Messenger
Rats
Reverse Transcriptase Polymerase Chain Reaction
Snare Proteins
Synaptosomal-associated Protein 25
Synaptotagmins
Syntaxin 1
Vesicle-associated Membrane Protein 2
Rights: aberto
Identifier DOI: /S0716-97602006000300016
Address: http://www.ncbi.nlm.nih.gov/pubmed/17106586
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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