Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196981
Type: Artigo de periódico
Title: Inhibition Of Specific Electron Transport Pathways Leads To Oxidative Stress And Decreased Candida Albicans Proliferation.
Author: Ruy, Fernando
Vercesi, Anibal E
Kowaltowski, Alicia J
Abstract: Candida parapsilosis mitochondria contain three respiratory chains: the classical respiratory chain (CRC), a secondary parallel chain (PAR) and an alternative oxidative pathway (AOX). We report here the existence of similar pathways in C. albicans. To observe the capacity of each pathway to sustain yeast growth, C. albicans cells were cultured in the presence of inhibitors of these pathways. Antimycin A and KCN totally abrogated yeast growth, while rotenone did not prevent proliferation. Furthermore, rotenone promoted only partial respiratory inhibition. Lower concentrations of KCN that promote partial inhibition of respiration did not inhibit yeast growth, while partial inhibition of respiration with antimycin A did. Similarly, AOX inhibitor BHAM decreased O(2) consumption slightly but completely stunted cell growth. Reactive oxygen species production and oxidized glutathione levels were enhanced in cells treated with antimycin A or BHAM, but not rotenone or KCN. These findings suggest that oxidative stress prevents C. albicans growth.
Subject: Antimycin A
Candida Albicans
Electron Transport
Glutathione
Hydroxamic Acids
Mitochondria
Mitochondrial Proteins
Oxidative Stress
Oxidoreductases
Oxygen Consumption
Plant Proteins
Reactive Oxygen Species
Rotenone
Uncoupling Agents
Rights: fechado
Identifier DOI: 10.1007/s10863-006-9012-7
Address: http://www.ncbi.nlm.nih.gov/pubmed/17053999
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_17053999.pdf353.46 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.