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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleFlow-cytometric Analysis Of Cytokine Production In Human Paracoccidioidomycosis.pt_BR
dc.contributor.authorMamoni, Ronei Lucianopt_BR
dc.contributor.authorBlotta, Maria Heloisa Souza Limapt_BR
unicamp.authorRonei Luciano Mamoni, Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), P.O. Box 6111, 13083-970 Campinas, SP, Brazil.pt_BR
unicamp.author.externalMaria Heloisa Souza Lima Blotta,pt
dc.subjectAdultpt_BR
dc.subjectCarrier Statept_BR
dc.subjectChemokinespt_BR
dc.subjectChemokines, Cxcpt_BR
dc.subjectChildpt_BR
dc.subjectCytokinespt_BR
dc.subjectFlow Cytometrypt_BR
dc.subjectHumanspt_BR
dc.subjectInterferon-gammapt_BR
dc.subjectInterleukin-10pt_BR
dc.subjectInterleukin-12pt_BR
dc.subjectLeukocytes, Mononuclearpt_BR
dc.subjectParacoccidioidespt_BR
dc.subjectParacoccidioidomycosispt_BR
dc.subjectReceptors, Cxcr3pt_BR
dc.subjectReceptors, Chemokinept_BR
dc.subjectTumor Necrosis Factor-alphapt_BR
dc.description.abstractHuman infection with Paracoccidioides brasiliensis may result in three major outcomes: paracoccidioidomycosis-infection (PI), which is observed in healthy carriers living in endemic areas and the adult form (AF) and juvenile form (JF) of the disease. In this study we proposed to examine the intracellular expression of IFN-gamma, TNF-alpha, IL-2, IL-10, IL-12, CXCL8, CXCL9 and CXCL10 by peripheral blood mononuclear cells (PBMC) of patients with the JF and AF of the disease, as well as of PI individuals stimulated with PMA plus ionomycin, LPS or anti-CD3 plus anti-CD28, by flow cytometry. The results showed that PI individuals present a higher percentage of cells producing IFN-gamma, TNF-alpha, IL-2, CXCL9 and CXCL10 when compared to AF and JF patients. IFN-gamma was predominantly detected in CD3(+)CD8(+) T cells, whereas IL-2 and TNF-alpha were mainly expressed in CD3(+)CD4(+) cells. Monocytes of PI individuals also presented higher expression of CD80 and lower expression of CD86 when compared to JF and AF patients, and higher expression of HLA-DR, only when compared to JF patients. These results indicate that the differential production of cytokines and chemokines, as well as the expression of co-stimulatory molecules involved in antigen presentation, may influence the outcome of PCM infection.en
dc.relation.ispartofCytokinept_BR
dc.relation.ispartofabbreviationCytokinept_BR
dc.date.issued2006-Augpt_BR
dc.identifier.citationCytokine. v. 35, n. 3-4, p. 207-16, 2006-Aug.pt_BR
dc.language.isoengpt_BR
dc.description.volume35pt_BR
dc.description.initialpage207-16pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1043-4666pt_BR
dc.identifier.doi10.1016/j.cyto.2006.08.005pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/17045486pt_BR
dc.date.available2015-11-27T13:05:56Z-
dc.date.accessioned2015-11-27T13:05:56Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:05:56Z (GMT). No. of bitstreams: 1 pmed_17045486.pdf: 504979 bytes, checksum: d00cf32a8553058500a9c1b045fc81b1 (MD5) Previous issue date: 2006en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196976-
dc.identifier.idPubmed17045486pt_BR
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