Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196976
Type: Artigo de periódico
Title: Flow-cytometric Analysis Of Cytokine Production In Human Paracoccidioidomycosis.
Author: Mamoni, Ronei Luciano
Blotta, Maria Heloisa Souza Lima
Abstract: Human infection with Paracoccidioides brasiliensis may result in three major outcomes: paracoccidioidomycosis-infection (PI), which is observed in healthy carriers living in endemic areas and the adult form (AF) and juvenile form (JF) of the disease. In this study we proposed to examine the intracellular expression of IFN-gamma, TNF-alpha, IL-2, IL-10, IL-12, CXCL8, CXCL9 and CXCL10 by peripheral blood mononuclear cells (PBMC) of patients with the JF and AF of the disease, as well as of PI individuals stimulated with PMA plus ionomycin, LPS or anti-CD3 plus anti-CD28, by flow cytometry. The results showed that PI individuals present a higher percentage of cells producing IFN-gamma, TNF-alpha, IL-2, CXCL9 and CXCL10 when compared to AF and JF patients. IFN-gamma was predominantly detected in CD3(+)CD8(+) T cells, whereas IL-2 and TNF-alpha were mainly expressed in CD3(+)CD4(+) cells. Monocytes of PI individuals also presented higher expression of CD80 and lower expression of CD86 when compared to JF and AF patients, and higher expression of HLA-DR, only when compared to JF patients. These results indicate that the differential production of cytokines and chemokines, as well as the expression of co-stimulatory molecules involved in antigen presentation, may influence the outcome of PCM infection.
Subject: Adult
Carrier State
Chemokines
Chemokines, Cxc
Child
Cytokines
Flow Cytometry
Humans
Interferon-gamma
Interleukin-10
Interleukin-12
Leukocytes, Mononuclear
Paracoccidioides
Paracoccidioidomycosis
Receptors, Cxcr3
Receptors, Chemokine
Tumor Necrosis Factor-alpha
Rights: fechado
Identifier DOI: 10.1016/j.cyto.2006.08.005
Address: http://www.ncbi.nlm.nih.gov/pubmed/17045486
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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