Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196934
Type: Artigo de periódico
Title: Fetal Rhd Genotyping From Maternal Plasma In A Population With A Highly Diverse Ethnic Background.
Author: Machado, Isabela Nelly
Castilho, Lilian
Pellegrino, Jordão
Barini, Ricardo
Abstract: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.
Subject: Brazil
Dna
Electrophoresis, Agar Gel
Ethnic Groups
Exons
Female
Fetal Blood
Genetic Variation
Genotype
Humans
Polymerase Chain Reaction
Pregnancy
Pregnancy Trimesters
Prenatal Diagnosis
Rh-hr Blood-group System
Sensitivity And Specificity
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/16967140
Date Issue: -1-Uns- -1
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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