Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196900
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleBiochemical And Genomic Analysis Of Sucrose Metabolism During Coffee (coffea Arabica) Fruit Development.pt_BR
dc.contributor.authorGeromel, Clarapt_BR
dc.contributor.authorFerreira, Lúcia Pirespt_BR
dc.contributor.authorGuerreiro, Sandra Maria Carmelopt_BR
dc.contributor.authorCavalari, Aline Andréiapt_BR
dc.contributor.authorPot, Davidpt_BR
dc.contributor.authorPereira, Luiz Filipe Protásiopt_BR
dc.contributor.authorLeroy, Thierrypt_BR
dc.contributor.authorVieira, Luiz Gonzaga Estevespt_BR
dc.contributor.authorMazzafera, Paulopt_BR
dc.contributor.authorMarraccini, Pierrept_BR
unicamp.authorClara Geromel, UNICAMP (Universidade Estadual de Campinas), Departamento de Fisiologia Vegetal, IB, CP 6109, 13083-970 Campinas, SP, Brazil.pt_BR
unicamp.author.externalLúcia Pires Ferreira,pt
unicamp.author.externalSandra Maria Carmelo Guerreiro,pt
unicamp.author.externalAline Andréia Cavalari,pt
unicamp.author.externalDavid Pot,pt
unicamp.author.externalLuiz Filipe Protásio Pereira,pt
unicamp.author.externalThierry Leroy,pt
unicamp.author.externalLuiz Gonzaga Esteves Vieira,pt
unicamp.author.externalPaulo Mazzafera,pt
unicamp.author.externalPierre Marraccini,pt
dc.subjectBiological Transportpt_BR
dc.subjectBlotting, Southernpt_BR
dc.subjectCarbonpt_BR
dc.subjectCarbon Dioxidept_BR
dc.subjectCarbon Radioisotopespt_BR
dc.subjectCoffeapt_BR
dc.subjectFructosept_BR
dc.subjectFruitpt_BR
dc.subjectGenome, Plantpt_BR
dc.subjectGenomicspt_BR
dc.subjectGlucosyltransferasespt_BR
dc.subjectMolecular Sequence Datapt_BR
dc.subjectPhylogenypt_BR
dc.subjectPlant Proteinspt_BR
dc.subjectProtein Isoformspt_BR
dc.subjectRna, Messengerpt_BR
dc.subjectSucrosept_BR
dc.subjectBeta-fructofuranosidasept_BR
dc.description.abstractSucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with (14)C-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (14)CO(2) showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 in perisperm, endosperm, and pericarp tissues: CaSUS1 mRNAs accumulated mainly during the early development of perisperm and endosperm, as well as during pericarp growing phases, whereas those of CaSUS2 paralleled sucrose synthase activity in the last weeks of pericarp and endosperm development. Taken together, these results indicate that sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit.en
dc.relation.ispartofJournal Of Experimental Botanypt_BR
dc.relation.ispartofabbreviationJ. Exp. Bot.pt_BR
dc.date.issued2006pt_BR
dc.identifier.citationJournal Of Experimental Botany. v. 57, n. 12, p. 3243-58, 2006.pt_BR
dc.language.isoengpt_BR
dc.description.volume57pt_BR
dc.description.firstpage3243-58pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0022-0957pt_BR
dc.identifier.doi10.1093/jxb/erl084pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/16926239pt_BR
dc.date.available2015-11-27T13:05:44Z-
dc.date.accessioned2015-11-27T13:05:44Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:05:44Z (GMT). No. of bitstreams: 1 pmed_16926239.pdf: 1290830 bytes, checksum: 40fb8a6a08f722bc090bbae4703e08e7 (MD5) Previous issue date: 2006en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196900-
dc.identifier.idPubmed16926239pt_BR
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