Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196899
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNeutrophil Migration In Mice Induced By A Mannose-binding Lectin Isolated From Annona Coriacea Seeds.pt_BR
dc.contributor.authorCoelho, Mirela Bpt_BR
dc.contributor.authorDesouza, Ivani Apt_BR
dc.contributor.authorFreire, Maria Graça Mpt_BR
dc.contributor.authorMarangoni, Sérgiopt_BR
dc.contributor.authorAntunes, Edsonpt_BR
dc.contributor.authorMacedo, Maria Lígia Rpt_BR
unicamp.authorMirela B Coelho, Departamento de Bioquímica, Instituto de Biologia (IB), Universidade Estadual de Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas (SP), Brazil. mbcoelho@yahoo.com.brpt_BR
unicamp.author.externalIvani A Desouza,pt
unicamp.author.externalMaria Graça M Freire,pt
unicamp.author.externalSérgio Marangoni,pt
unicamp.author.externalEdson Antunes,pt
unicamp.author.externalMaria Lígia R Macedo,pt
dc.subjectAnimalspt_BR
dc.subjectAnnonapt_BR
dc.subjectChemotaxis, Leukocytept_BR
dc.subjectDihydropyridinespt_BR
dc.subjectDose-response Relationship, Drugpt_BR
dc.subjectLeukocytes, Mononuclearpt_BR
dc.subjectMalept_BR
dc.subjectMannosept_BR
dc.subjectMannose-binding Lectinpt_BR
dc.subjectMicept_BR
dc.subjectNeutrophilspt_BR
dc.subjectPeritoneal Cavitypt_BR
dc.subjectPlant Extractspt_BR
dc.subjectPyrazolespt_BR
dc.subjectSeedspt_BR
dc.subjectSulfonamidespt_BR
dc.description.abstractA novel 14-kDa lectin from Annona coriacea seeds (ACLEC) with hemagglutinating activity on erythrocytes has been recently described. Since plant lectins are known to present inflammatory activity, this study aimed to investigate the leukocyte migration induced by ACLEC, and inflammatory mediators involved in this phenomenon. Male Swiss mice were intraperitoneally injected with ACLEC (3-100 microg/cavity), and at 4-96 h thereafter the leukocyte counts in peritoneal lavage fluid were evaluated. ACLEC induced a dose-dependent neutrophil accumulation, reaching maximal responses at 16 h after injection (approximately 40-fold increase for 30 microg/cavity). Significant accumulation of mononuclear cells was observed at 72 h (2.7-fold increase). The carbohydrate mannose nearly abolished the neutrophil influx, whereas sucrose, glucose and galactose had no effect. Dexamethasone, the cyclooxygenase-2 (COX-2) inhibitor celecoxib and the Platelet activating factor (PAF) receptor antagonist PCA4248 significantly reduced ACLEC-induced neutrophil influx. The tachykinin NK(1) antagonist SR140333, the tachykinin NK(2) antagonist SR48968, the non-selective NO inhibitor L-NAME, the selective inducible NOS inhibitor aminoguanidine and the lypoxygenase inhibitor AA861 all failed to modify the ACLEC-induced responses. In conclusion, ACLEC is able to attract neutrophils into the mice peritoneal cavity by mechanisms involving interactions of the lectin with cell-specific mannose recognition, leading to the release of COX-2-derived mediators and PAF.en
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinologypt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.date.issued2006-Octpt_BR
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology. v. 48, n. 5, p. 529-35, 2006-Oct.pt_BR
dc.language.isoengpt_BR
dc.description.volume48pt_BR
dc.description.firstpage529-35pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.doi10.1016/j.toxicon.2006.07.001pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/16926040pt_BR
dc.date.available2015-11-27T13:05:44Z-
dc.date.accessioned2015-11-27T13:05:44Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:05:44Z (GMT). No. of bitstreams: 1 pmed_16926040.pdf: 220403 bytes, checksum: ca88582857fecf2722eaec1d2fa44479 (MD5) Previous issue date: 2006en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196899-
dc.identifier.idPubmed16926040pt_BR
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