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Type: Artigo de periódico
Title: Acetylcholine Receptors And Nerve Terminal Distribution At The Neuromuscular Junction Of Long-term Regenerated Muscle Fibers.
Author: Marques, Maria Julia
Mendes, Zarif T R
Minatel, Elaine
Santo Neto, Humberto
Abstract: Mdx mice are deficient in dystrophin and show muscle fiber regeneration. Changes in the distribution of acetylcholine receptors have been reported at the neuromuscular junction of mdx mice and may be a consequence of muscle fiber regeneration. In this study, we examined whether the distribution of receptors was still altered in long-term, regenerated muscle fibers from C57Bl/10 mice. The left sternomastoid muscle of adult mice was injected with 60 microl of lidocaine hydrochloride to induce muscle degeneration-regeneration. In some mice, the sternomastoid muscle was denervated at the time of lidocaine injection. After 90 and 150 days, the nicotinic acetylcholine receptors were labeled with rhodamine-alpha-bungarotoxin for confocal microscopy. At both intervals studied, the receptors were distributed in spots. In denervated-regenerated fibers, the receptors were distributed as regular branches similar to denervated muscles without lidocaine treatment. These findings suggested that nerve-dependent mechanisms were involved in the changes in receptor distribution seen in regenerated muscle fibers after lidocaine treatment, and that a similar phenomenon could explain the changes in receptor distribution seen in dystrophic muscle fibers.
Subject: Animals
Mice, Inbred C57bl
Microscopy, Confocal
Muscle Fibers, Skeletal
Muscle, Skeletal
Muscular Dystrophies
Neuromuscular Junction
Presynaptic Terminals
Receptors, Nicotinic
Synaptic Transmission
Time Factors
Citation: Journal Of Neurocytology. v. 34, n. 6, p. 387-96, 2005-Dec.
Rights: fechado
Identifier DOI: 10.1007/s11068-006-8725-1
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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