Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196860
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCytotoxic Activity Of Violacein In Human Colon Cancer Cells.pt_BR
dc.contributor.authorde Carvalho, Daniela Dpt_BR
dc.contributor.authorCosta, Fabio T Mpt_BR
dc.contributor.authorDuran, Nelsonpt_BR
dc.contributor.authorHaun, Marcelapt_BR
unicamp.authorDaniela D de Carvalho, Departamento de Bioquímica, IB, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. ddc@unicamp.brpt_BR
unicamp.author.externalFabio T M Costa,pt
unicamp.author.externalNelson Duran,pt
unicamp.author.externalMarcela Haun,pt
dc.subjectApoptosispt_BR
dc.subjectCaco-2 Cellspt_BR
dc.subjectCalciumpt_BR
dc.subjectCaspase 3pt_BR
dc.subjectCell Deathpt_BR
dc.subjectCell Survivalpt_BR
dc.subjectColonic Neoplasmspt_BR
dc.subjectCytochromes Cpt_BR
dc.subjectEnzyme Activationpt_BR
dc.subjectHt29 Cellspt_BR
dc.subjectHumanspt_BR
dc.subjectIndicators And Reagentspt_BR
dc.subjectIndolespt_BR
dc.subjectReactive Oxygen Speciespt_BR
dc.subjectTetrazolium Saltspt_BR
dc.subjectThiazolespt_BR
dc.description.abstractSeveral studies have shown that violacein, a purple pigment extracted from Chromobacterium violaceum, is capable to induce apoptosis in a variety of cancer cells, including those leukemia cell lines. Herein, we examined the effects of violacein on reactive oxygen species (ROS) production during the apoptotic colon cancer cell death. We demonstrate that violacein mediates ROS production followed by activation of Caspase-3, release of cytochrome c, and calcium release to citosol in Caco-2 cells. Moreover, presence of ROS scavengers such as N-acetyl-cysteine (NAC) diminishes ROS cytotoxicity induced by violacein in Caco-2 cells, indicating that violacein mediates cellular critical mechanisms in the triggering of apoptotic tumor cell death. These data also imply that violacein-induced ROS are collectively key mediators of mitochondrial membrane collapse, leading to cytochrome c release, and culminating in tumor apoptosis. Unlike in Caco-2 cells, violacein was incapable of increasing ROS levels in HT29 cells, suggesting the existence of violacein cell-type specific mechanisms. Those findings bring light to the violacein cytotoxic mechanism studies, indicating that oxidative stress play a role in the violacein-induced cytotoxicity.en
dc.relation.ispartofToxicology In Vitro : An International Journal Published In Association With Bibrapt_BR
dc.relation.ispartofabbreviationToxicol In Vitropt_BR
dc.date.issued2006-Decpt_BR
dc.identifier.citationToxicology In Vitro : An International Journal Published In Association With Bibra. v. 20, n. 8, p. 1514-21, 2006-Dec.pt_BR
dc.language.isoengpt_BR
dc.description.volume20pt_BR
dc.description.firstpage1514-21pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0887-2333pt_BR
dc.identifier.doi10.1016/j.tiv.2006.06.007pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/16889929pt_BR
dc.date.available2015-11-27T13:05:39Z-
dc.date.accessioned2015-11-27T13:05:39Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:05:39Z (GMT). No. of bitstreams: 1 pmed_16889929.pdf: 475878 bytes, checksum: 87351689e678ecfd2fc938dbd9a7ffef (MD5) Previous issue date: 2006en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196860-
dc.identifier.idPubmed16889929pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_16889929.pdf464.72 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.