Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196854
Type: Artigo de periódico
Title: Genetic Variability And Chromosome-length Polymorphisms Of The Witches' Broom Pathogen Crinipellis Perniciosa From Various Plant Hosts In South America.
Author: Rincones, Johana
Mazotti, Gabriel D
Griffith, Gareth W
Pomela, Alan
Figueira, Antonio
Leal, Gildemberg A
Queiroz, Marisa V
Pereira, Jorge F
Azevedo, Ricardo A
Pereira, Gonçalo A G
Meinhardt, Lyndel W
Abstract: Crinipellis perniciosa has been classified into at least four known biotypes associated with members of unrelated plant families. In this study, genetic variability is shown for 27 C (Cacao), 4 S (Solanum), and 7 L biotype (Liana) isolates of C. perniciosa collected from different regions of Brazil and South America. The objective was to investigate the genetic variability of the pathogen in the cacao-producing region of Bahia, Brazil, and elsewhere, through microsatellite analysis, and attempt to identify possible correlations between host specificity and electrophoretic karyotypes. The PCR-banding patterns were found to vary both within and between the different biotypes, and a correlation was established between the PCR-banding patterns and the chromosomal-banding patterns of each isolate. Microsatellite and chromosomal patterns among all of the L and S biotype isolates were distinctly different from the C biotypes analysed. A higher degree of genetic and chromosomal variability was found among C biotype isolates from the Amazon in comparison with C biotype isolates from Bahia, which seems to be comprised of only two main genotypes. This finding has important implications to the current cacao-breeding programme in Brazil.
Subject: Agaricales
Angiosperms
Blotting, Southern
Chromosomes, Fungal
Dna, Fungal
Dna, Ribosomal
Electrophoresis, Gel, Pulsed-field
Genes, Fungal
Genetic Variation
Karyotyping
Microsatellite Repeats
Mycological Typing Techniques
Polymerase Chain Reaction
Polymorphism, Genetic
South America
Rights: fechado
Identifier DOI: 10.1016/j.mycres.2006.05.002
Address: http://www.ncbi.nlm.nih.gov/pubmed/16876701
Date Issue: 2006
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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