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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDetermination Of Glutathione In Hemolysed Erythrocyte With Amperometric Sensor Based On Ttf-tcnq.pt_BR
dc.contributor.authorCalvo-Marzal, Percypt_BR
dc.contributor.authorChumbimuni-Torres, Karin Ypt_BR
dc.contributor.authorHöehr, Nelci Fenaltipt_BR
dc.contributor.authorKubota, Lauro Tpt_BR
unicamp.authorPercy Calvo-Marzal, Instituto de Química-UNICAMP, Departamento de Química Analítica. P.O. Box 6154, 13083-970 Campinas-SP, Brazil.pt_BR
unicamp.author.externalKarin Y Chumbimuni-Torres,pt
unicamp.author.externalNelci Fenalti Höehr,pt
unicamp.author.externalLauro T Kubota,pt
dc.subjectBiosensing Techniquespt_BR
dc.subjectElectrochemistrypt_BR
dc.subjectErythrocytespt_BR
dc.subjectGlutathionept_BR
dc.subjectHemolysispt_BR
dc.subjectHeterocyclic Compoundspt_BR
dc.subjectHumanspt_BR
dc.subjectIndicators And Reagentspt_BR
dc.subjectNitrilespt_BR
dc.subjectReproducibility Of Resultspt_BR
dc.subjectSpectrophotometrypt_BR
dc.description.abstractGSH has a relevant role in human metabolism as an indicator of disease risks. An amperometric sensor for glutathione (GSH) determination is described as an alternative method featuring simple construction procedure and short time analysis. The developed sensor was used to determine glutathione at low potential using a TTF-TCNQ complex. The sensor exhibits a linear response range from 5 to 340 micromol/l under applied potential of 200 mV vs. SCE. The sensitivity and detection limit were 90.1 microA l/mmol cm(2) and 0.3 micromol/l, respectively. When the sensor was tested in hemolysed erythrocyte samples for GSH determination, a good correlation in results was observed between the sensor and the spectrophotometric method. The sensor showed recovery values between 98% and 102%.en
dc.relation.ispartofClinica Chimica Acta; International Journal Of Clinical Chemistrypt_BR
dc.relation.ispartofabbreviationClin. Chim. Actapt_BR
dc.date.issued2006-Seppt_BR
dc.identifier.citationClinica Chimica Acta; International Journal Of Clinical Chemistry. v. 371, n. 1-2, p. 152-8, 2006-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume371pt_BR
dc.description.firstpage152-8pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0009-8981pt_BR
dc.identifier.doi10.1016/j.cca.2006.03.006pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/16650398pt_BR
dc.date.available2015-11-27T13:05:24Z-
dc.date.accessioned2015-11-27T13:05:24Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:05:24Z (GMT). No. of bitstreams: 1 pmed_16650398.pdf: 162979 bytes, checksum: 314e81cbfb5535cc1b5dc1a3e5627540 (MD5) Previous issue date: 2006en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196749-
dc.identifier.idPubmed16650398pt_BR
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