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Type: Artigo de periódico
Title: Evaluation Of Ida-peva Hollow Fiber Membrane Metal Ion Affinity Chromatography For Purification Of A Histidine-tagged Human Proinsulin.
Author: de Aquino, Luciana Cristina Lins
de Sousa, Heloisa Ribeiro Tunes
Miranda, Everson Alves
Vilela, Luciano
Bueno, Sônia Maria Alves
Abstract: Inabilities to process particulate material and to allow the use of high flow rates are limitations of conventional chromatography. Membranes have been suggested as matrix for affinity separation due to advantages such as allowing high flow rates and low-pressure drops. This work evaluated the feasibility of using an iminodiacetic acid linked poly(ethylenevinyl alcohol) membrane in the immobilized metal ion affinity chromatography (IMAC) purification of a human proinsulin(His)(6) of an industrial insulin production process. The screening of metal ions showed Ni(2+) as metal with higher selectivity and capacity among the Cu(2+), Ni(2+), Zn(2+) and Co(2+). The membrane showed to be equivalent to conventional chelating beads in terms of selectivity and had a lower capacity (3.68 mg/g versus 12.26 mg/g). The dynamic adsorption capacity for human proinsulin(His)(6) was unaffected by the mode of operation (dead-end and cross-flow filtration).
Subject: Adsorption
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Membranes, Artificial
Citation: Journal Of Chromatography. B, Analytical Technologies In The Biomedical And Life Sciences. v. 834, n. 1-2, p. 68-76, 2006-Apr.
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2006.02.015
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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