Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196591
Type: Artigo de periódico
Title: Effects Of Hyperbaric Oxygen On Leishmania Amazonensis Promastigotes And Amastigotes.
Author: Arrais-Silva, Wagner Weber
Collhone, Marcelle Carolina
Ayres, Diana Copi
de Souza Souto, Paula Cristina
Giorgio, Selma
Abstract: In the present study, we evaluated the effects of hyperbaric oxygen (HBO) exposure in both Leishmania amazonensis life stages (promastigotes and amastigotes) and on macrophage cultures infected with the parasite. HBO treatment protocols, which can be tolerated by humans and animals, induced irreversible metabolic damage and affected parasite morphology, growth and ability to transform. The observation that the antioxidant N-acetylcysteine (NAC) prevents some of these deleterious effects indicated an involvement of oxidative stress during parasite HBO exposure. In addition, HBO exposed L. amazonensis-infected macrophage cultures showed reduction of the percentage of infected cells and of the number of intracellular parasites per cell. Thus, the demonstration that HBO, a therapy used in the management of different diseases, is toxic for both L. amazonensis life stages and can alter macrophage susceptibility to the infection encourages further studies of this therapy in animal models of Leishmania infection.
Subject: Animals
Cells, Cultured
Humans
Hyperbaric Oxygenation
Leishmania
Leishmaniasis
Life Cycle Stages
Macrophages, Peritoneal
Mice
Mice, Inbred Balb C
Oxygen
Rights: fechado
Identifier DOI: 10.1016/j.parint.2004.07.002
Address: http://www.ncbi.nlm.nih.gov/pubmed/15710544
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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