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Type: Artigo de periódico
Title: Aflatoxin Screening By Maldi-tof Mass Spectrometry.
Author: Ramos Catharino, Rodrigo
de Azevedo Marques, Lygia
Silva Santos, Leonardo
Baptista, Antonio S
Glória, Eduardo M
Calori-Domingues, Maria A
Facco, Elizete M P
Eberlin, Marcos N
Abstract: Efficient detection of aflatoxins B1, B2, G1, and G2 has been performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a UV-absorbing ionic liquid matrix to obtain matrix-free mass spectra and addition of NaCl to enhance sensitivity via Na+ cationization. Using ionic alpha-cyano-4-hydroxycinnamic acid (Et3N-alpha-CHCA) as the matrix, matrix-free mass spectra in the m/z range of interest are acquired, and the B1, B2, G1, and G2 aflatoxins are readily detected with an LOD as low as 50 fmol. The technique is fast, requires little sample preparation and no derivatization or chromatographic separation, and seems therefore to be suitable for high-throughput aflatoxin screening. It should be easily extended to other micotoxins and provide an attractive technique to control the quality of major crops subjected to huge world commercial trades such as peanuts, corn, and rice as well as to monitor bioterrorism threats by micotoxin poisoning.
Subject: Aflatoxins
Arachis Hypogaea
Food Contamination
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Citation: Analytical Chemistry. v. 77, n. 24, p. 8155-7, 2005-Dec.
Rights: fechado
Identifier DOI: 10.1021/ac051111p
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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