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Type: | Artigo de periódico |
Title: | Cadmium Chloride-induced Oxidative Stress In Skeletal Muscle Cells In Vitro. |
Author: | Yano, Claudia Lumy Marcondes, Maria Cristina Cintra Gomes |
Abstract: | The effects of cadmium chloride (CdCl(2)) on oxidative stress in the skeletal muscle cell line C(2)C(12) were investigated. Myoblast cells that differentiated into myotubes were treated with CdCl(2) (1, 3, 5, 7.5, 10, and 12.5 microM) for 24, 48, and 72 h. Subsequent assay of cell homogenates for MTT (3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide) reduction, neutral red uptake and nucleic acid content showed that cadmium was toxic to C(2)C(12) cells in a concentration-dependent manner. Glutathione-S-transferase activity (nmol microg of protein(-1) min(-1)) was increased with 1 and 3 microM CdCl(2) (36.9 +/- 5.6 and 32.1 +/- 6.0, respectively) compared to control cells (21.8 +/- 1.5), but decreased at higher concentrations (7.5 microM = 15.9 +/- 3.3, 10 microM = 15.9 +/- 4.6, and 12.5 microM = 10.5 +/- 2.8). An increase in malondialdehyde content (nmol microg of protein(-1)), especially at high CdCl(2) concentrations (control = 7.3 +/- 0.5; CdCl(2): 7.5 microM = 11.2 +/- 3.1, 10 microM = 14.6 +/- 3.8, and 12.5 microM = 20.5 +/- 6.5) indicated that there was enhanced lipid peroxidation. Light and scanning electron microscopy showed that there was a concentration-dependent loss of adherent cells and the formation of vesicles indicative of cell death. These results indicated that CdCl(2) increased oxidative stress in C(2)C(12) cells, and this stress probably compromised cell adhesion and the cellular antioxidant defense mechanisms. |
Subject: | Animals Antioxidants Cadmium Chloride Cell Adhesion Cell Death Cell Differentiation Cell Line Cell Survival Dose-response Relationship, Drug Glutathione Transferase In Vitro Techniques Lipid Peroxidation Mice Microscopy, Electron, Scanning Muscle, Skeletal Oxidative Stress Signal Transduction Tetrazolium Salts Thiazoles Time Factors |
Citation: | Free Radical Biology & Medicine. v. 39, n. 10, p. 1378-84, 2005-Nov. |
Rights: | fechado |
Identifier DOI: | 10.1016/j.freeradbiomed.2005.07.001 |
Address: | http://www.ncbi.nlm.nih.gov/pubmed/16257647 |
Date Issue: | 2005 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
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pmed_16257647.pdf | 767.47 kB | Adobe PDF | View/Open |
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