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Type: Artigo de periódico
Title: Volume Of Extracellular Polymeric Substance Coverage Of Individual Acidithiobacillus Ferrooxidans Bacterium Measured By Atomic Force Microscopy.
Author: Teschke, Omar
Abstract: The Acidithiobacillus ferrooxidans response to stress associated with the drying process is known to be the production of extracellular polymeric substance (EPS) coverage. Here, samples of A. ferrooxidans suspensions grown in 1.8 pH and 3.0 pH and dried on mica and silicon are shown to form a structure of isolated bacteria. Individual bacteria coverage patterns were imaged by atomic force microscopy (AFM) on hydrophobic (silicon) and hydrophilic (mica) substrates. A comparison of images of covered and uncovered bacteria establish the volume of individual EPS coverage. The EPS production for bacteria on hydrophobic substrates shows a substantial decrease (a factor of 30) in the EPS volume per bacterium when compared with the one on hydrophilic substrates. Shape and volume determination of EPS structures on bacteria as a function of hydrophobicity or hydrophilicity of the substrate may help to determine the functions of EPS on bacterial aggregates.
Subject: Acidithiobacillus
Aluminum Silicates
Culture Media
Hydrogen-ion Concentration
Hydrophobic And Hydrophilic Interactions
Microscopy, Atomic Force
Polysaccharides, Bacterial
Citation: Microscopy Research And Technique. v. 67, n. 6, p. 312-6, 2005-Aug.
Rights: fechado
Identifier DOI: 10.1002/jemt.20203
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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