Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196382
Type: Artigo de periódico
Title: Protective Effect Of Melatonin On Rotenone Plus Ca2+-induced Mitochondrial Oxidative Stress And Pc12 Cell Death.
Author: Sousa, Solange C
Castilho, Roger F
Abstract: Chronic systemic inhibition of mitochondrial respiratory chain complex I by rotenone causes nigrostriatal dopaminergic degeneration in rats, producing an in vivo experimental model of Parkinson's disease. We recently showed that micromolar Ca2+ concentrations strongly stimulate the release of reactive oxygen species in rotenone-treated isolated rat brain mitochondria. In the present work, we show that the natural antioxidant melatonin inhibits Ca2+ plus rotenone-induced oxidative stress in isolated rat brain mitochondria. In addition, the Ca2+ ionophore A23187 strongly potentiates rotenone-induced death of intact cultured pheochromocytoma (PC12) cells, in a mechanism sensitive to melatonin. Moreover, melatonin inhibits the detection of reactive oxygen species release in PC12 cells treated with rotenone plus A23187. Melatonin does not alter free Ca2+ concentrations or the inhibitory effect of rotenone on mitochondrial complex I. We conclude that micromolar Ca2+ concentrations stimulate neuronal cell death induced by mitochondrial complex I inhibition in a mechanism involving oxidative stress, preventable by the antioxidant melatonin.
Subject: Adenosine Triphosphate
Animals
Antioxidants
Brain
Calcimycin
Calcium
Cytoplasm
Cytosol
Dose-response Relationship, Drug
Electron Transport Complex I
Ionophores
Melatonin
Mitochondria
Models, Chemical
Oxidative Stress
Oxygen
Pc12 Cells
Rats
Reactive Oxygen Species
Rotenone
Time Factors
Uncoupling Agents
Rights: fechado
Identifier DOI: 10.1089/ars.2005.7.1110
Address: http://www.ncbi.nlm.nih.gov/pubmed/16115015
Date Issue: -1-Uns- -1
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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