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http://repositorio.unicamp.br/jspui/handle/REPOSIP/196382
Type: | Artigo de periódico |
Title: | Protective Effect Of Melatonin On Rotenone Plus Ca2+-induced Mitochondrial Oxidative Stress And Pc12 Cell Death. |
Author: | Sousa, Solange C Castilho, Roger F |
Abstract: | Chronic systemic inhibition of mitochondrial respiratory chain complex I by rotenone causes nigrostriatal dopaminergic degeneration in rats, producing an in vivo experimental model of Parkinson's disease. We recently showed that micromolar Ca2+ concentrations strongly stimulate the release of reactive oxygen species in rotenone-treated isolated rat brain mitochondria. In the present work, we show that the natural antioxidant melatonin inhibits Ca2+ plus rotenone-induced oxidative stress in isolated rat brain mitochondria. In addition, the Ca2+ ionophore A23187 strongly potentiates rotenone-induced death of intact cultured pheochromocytoma (PC12) cells, in a mechanism sensitive to melatonin. Moreover, melatonin inhibits the detection of reactive oxygen species release in PC12 cells treated with rotenone plus A23187. Melatonin does not alter free Ca2+ concentrations or the inhibitory effect of rotenone on mitochondrial complex I. We conclude that micromolar Ca2+ concentrations stimulate neuronal cell death induced by mitochondrial complex I inhibition in a mechanism involving oxidative stress, preventable by the antioxidant melatonin. |
Subject: | Adenosine Triphosphate Animals Antioxidants Brain Calcimycin Calcium Cytoplasm Cytosol Dose-response Relationship, Drug Electron Transport Complex I Ionophores Melatonin Mitochondria Models, Chemical Oxidative Stress Oxygen Pc12 Cells Rats Reactive Oxygen Species Rotenone Time Factors Uncoupling Agents |
Citation: | Antioxidants & Redox Signaling. v. 7, n. 9-10, p. 1110-6 |
Rights: | fechado |
Identifier DOI: | 10.1089/ars.2005.7.1110 |
Address: | http://www.ncbi.nlm.nih.gov/pubmed/16115015 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
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pmed_16115015.pdf | 327.09 kB | Adobe PDF | View/Open |
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