Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196364
Type: Artigo de periódico
Title: Modulation Of Smooth Muscle Cell Function: Morphological Evidence For A Contractile To Synthetic Transition In The Rat Ventral Prostate After Castration.
Author: Vilamaior, Patricia Simone Leite
Taboga, Sebastião Roberto
Carvalho, Hernandes F
Abstract: In this study, we evaluated the involvement of rat ventral prostate smooth muscle cells (SMC) in secretory activity and whether this function is modulated after castration. Cell morphology was examined at both light and electron microscopy levels and the organelles involved in secretory function were labeled by the zinc-iodide-osmium (ZIO) method at the ultrastructural level and their volume density was determined by stereology. Castration resulted in marked changes of the SMC, which adopted a spinous aspect and abandoned the layered arrangement observed in the prostates of non-castrated rats. The volume density of ZIO reactive organelles increased progressively after castration, reaching significantly higher levels 21 days after castration. Since previous studies have demonstrated that SMC express SMC markers (even 21 days after castration) and are able to respond to adrenergic stimulation, we concluded that differentiated SMC are able to shift from a predominantly contractile to a more synthetic phenotype without changing their differentiation status.
Subject: Androgens
Animals
Antigens, Differentiation
Cell Differentiation
Endoplasmic Reticulum
Golgi Apparatus
Iodides
Male
Mitochondria, Muscle
Muscle Contraction
Myocytes, Smooth Muscle
Orchiectomy
Organ Size
Osmium Tetroxide
Phenotype
Prostate
Rats
Rats, Wistar
Secretory Vesicles
Zinc Compounds
Rights: fechado
Identifier DOI: 10.1016/j.cellbi.2005.05.006
Address: http://www.ncbi.nlm.nih.gov/pubmed/16085435
Date Issue: 2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_16085435.pdf622.75 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.