Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196361
Type: Artigo de periódico
Title: In Vivo Apoptosis Induction And Reduction Of Infectivity By An Autographa Californica Multiple Nucleopolyhedrovirus P35(-) Recombinant In Hemocytes From The Velvet Bean Caterpillar Anticarsia Gemmatalis (hübner) (lepidoptera: Noctuidae).
Author: da Silveira, Eni Braga
Cordeiro, Bruno Arrivabene
Ribeiro, Bergmann Morais
Báo, Sônia Nair
Abstract: Baculoviruses have long been shown to regulate apoptosis in cultured insect cells. Recently, this phenomenon was also reported to occur in vivo, reinforcing the importance of apoptosis in insect immunity against viruses. The vP35del virus, an Autographa californica multiple nucleopolyhedrovirus (AcMNPV) recombinant, was previously shown to induce apoptosis in Anticarsia gemmatalis cultured cells. In order to verify the AcMNPV interaction with hemocytes, apoptosis induction in vivo and its effects upon infectivity, we studied the course of intrahemocoelic infection of recombinant viruses (vHSGFP and vHSGFP/P35del) in A. gemmatalis larvae. Insect development and mortality were monitored and infection progress was followed by light, fluorescence and electron microscopy. For all doses tested, vHSGFP/P35del caused lower mortality than vHSGFP. Mortality of 95% occurred with a dose of 4x10(6) PFUs of vHSGFP, which was reduced to 60% for vHSGFP/P35del. GFP expression was first observed at 3 h p.i. for the two viruses, increasing for vHSGFP (40% at 120 h p.i.) and decreasing for vHSGFP/P35del (0% at 120 h p.i.). The virus vHSGFP/P35del induced apoptosis in hemocytes, with some budded virus being produced, and fragmented cells were observed between 24 and 72 h p.i. The recombinant vHSGFP induced typical wild-type cytopathic effects, with low production of occluded viruses until 120 h p.i. Plasmatocytes and granular hemocytes type 1 were the hemocytes most susceptible to both viruses. For these experimental conditions, we concluded that A. gemmatalis is a semi-permissive host to AcMNPV; moreover, apoptosis reduces AcMNPV infectivity and the p35 gene is essential for blocking apoptosis in this system.
Subject: Animals
Apoptosis
Cells, Cultured
Hemocytes
Hemolymph
Larva
Microscopy, Electron, Transmission
Moths
Nucleopolyhedrovirus
Recombination, Genetic
Viral Proteins
Rights: fechado
Identifier DOI: 10.1016/j.resmic.2005.06.001
Address: http://www.ncbi.nlm.nih.gov/pubmed/16081248
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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