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Type: Artigo de periódico
Title: A Monoclonal Antibody-based Elisa Allows Discrimination Between Responses Induced By Bovine Herpesvirus Subtypes 1 (bohv-1.1) And 2 (bohv-1.2).
Author: Spilki, Fernando Rosado
Esteves, Paulo Augusto
da Silva, Alessandra D'Avila
Franco, Ana Cláudia
Rijsewijk, Frans A M
Roehe, Paulo Michel
Abstract: Bovine herpesvirus type 1 (BoHV-1) has distinct subtypes according to genomic characterization. Immune responses induced by BoHV-1 subtype 1 (BoHV-1.1) are not distinguishable from those induced by BoHV-1 subtype 2 (BoHV-1.2) through conventional serological methods. In the present report, an enzyme linked immunosorbent assay is described that allows discrimination between immune responses in cattle immunized with either subtype, based on a monoclonal antibody that recognizes specifically the amino-terminal region of glycoprotein C (gC) on BoHV-1.1 strains, thus not reacting with BoHV-1.2a. The test displayed a sensitivity of 92%, specificity of 90% and a good correlation with serum neutralization tests on samples from BoHV-1.1-immunized calves (kappa = 0.799). The test may be useful to provide new insights into the roles played by each of these two subtypes in the epidemiology of BoHV-1 infections.
Subject: Animals
Antibodies, Monoclonal
Antibodies, Viral
Antibody Specificity
Enzyme-linked Immunosorbent Assay
Herpes Simplex
Herpesviridae Infections
Herpesvirus 1, Bovine
Herpesvirus 2, Bovine
Infectious Bovine Rhinotracheitis
Sensitivity And Specificity
Viral Proteins
Citation: Journal Of Virological Methods. v. 129, n. 2, p. 191-3, 2005-Nov.
Rights: fechado
Identifier DOI: 10.1016/j.jviromet.2005.06.008
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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