Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196320
Type: Artigo de periódico
Title: Nitrite As The Major Source Of Nitric Oxide Production By Arabidopsis Thaliana In Response To Pseudomonas Syringae.
Author: Modolo, Luzia V
Augusto, Ohara
Almeida, Irene M G
Magalhaes, Jose R
Salgado, Ione
Abstract: The origin of nitric oxide (*NO) in plants is unclear and an *NO synthase (NOS)-like enzyme and nitrate reductase (NR) are claimed as potential sources. Here we used wild-type and NR-defective double mutant plants to investigate *NO production in Arabidopsis thaliana in response to Pseudomonas syringae pv maculicola. NOS activity increased substantially in leaves inoculated with P. syringae. However, electron paramagnetic resonance experiments showed a much higher *NO formation that was dependent on nitrite and mitochondrial electron transport rather than on arginine or nitrate. Overall, these results indicate that NOS, NR and a mitochondrial-dependent nitrite-reducing activity cooperate to produce *NO during A. thaliana-P. syringae interaction.
Subject: Arabidopsis
Electron Spin Resonance Spectroscopy
Mutation
Nitrate Reductase
Nitrate Reductases
Nitric Oxide
Nitric Oxide Synthase
Nitrites
Plant Leaves
Pseudomonas Syringae
Rights: fechado
Identifier DOI: 10.1016/j.febslet.2005.05.078
Address: http://www.ncbi.nlm.nih.gov/pubmed/15978583
Date Issue: 2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_15978583.pdf208.89 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.