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dc.typeArtigo de periódicopt_BR
dc.titleIn Vitro Effect Of The Phoneutria Nigriventer Spider Venom On Cell Viability, Paracellular Barrier Function And Transcellular Transport In Cultured Cell Lines.pt_BR
dc.contributor.authorde Paula Le Sueur, Lucianapt_BR
dc.contributor.authorCollares-Buzato, Carla Beatrizpt_BR
dc.contributor.authorKalapothakis, Evanguedespt_BR
dc.contributor.authorda Cruz-Höfling, Maria Alicept_BR
unicamp.authorLuciana de Paula Le Sueur, Departamento de Histologia e Embriologia, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP), CEP 13083-970 Campinas, SP, Brazil.pt_BR Beatriz Collares-Buzato,pt Kalapothakis,pt Alice da Cruz-Höfling,pt
dc.subjectBiological Transportpt_BR
dc.subjectCell Linept_BR
dc.subjectCell Survivalpt_BR
dc.subjectElectric Impedancept_BR
dc.subjectHorseradish Peroxidasept_BR
dc.subjectMembrane Proteinspt_BR
dc.subjectNeutral Redpt_BR
dc.subjectSpider Venomspt_BR
dc.description.abstractPhoneutria nigriventer spider venom (PNV) induces, in rats, local edema as result of an increased vascular permeability, as well as causes blood-brain barrier (BBB) breakdown by altering transendothelial transport routes in hippocampal microvessels. In this work we investigated the in vitro effects of PNV on cell viability and cellular transport routes using three cell lines, the ECV304 endothelial-, the C6 glioma- and the MDCK epithelial cells. We showed that PNV (14.6 and 292 microg crude venom/ml culture medium) had no direct cytotoxic effect on both the ECV304 and the MDCK cell lines but slightly reduced the viability of C6 glioma cells (P<0.05) at the highest concentration, as revealed by the cellular neutral red uptake assay. The PNV effects on cell transport were evaluated in MDCK cell line. PNV seems do not cause any disturbance in the paracellular barrier function of the cultured MDCK cells, as shown by the lack of a significant change in the distribution and expression of the junctional proteins, ZO-1, occludin, E-cadherin and the cytoskeletal F-actin. In contrast, PNV-treated MDCK monolayers showed an enhancement in the transepithelial electrical resistance and a tendency towards an increased occludin expression. In addition, the PNV significantly increased the apical endocytosis of HRP, which was not followed by an equivalent exocytosis at the basal side, as revealed by biochemical and ultrastructural methods. We conclude that the venom of P. nigriventer displays a relatively low cytotoxicity in vitro as well as activates directly the endocytic transport pathway in MDCK cells without disrupting the paracellular route.en
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinologypt_BR
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology. v. 46, n. 2, p. 130-41, 2005-Aug.pt_BR
dc.description.provenanceMade available in DSpace on 2015-11-27T13:02:11Z (GMT). No. of bitstreams: 1 pmed_15949833.pdf: 692719 bytes, checksum: b63a605547f53a8e4b0571efbbac03df (MD5) Previous issue date: 2005en
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