Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196250
Type: Artigo de periódico
Title: Evidence For Two Atypical Conformations Of Beta-adrenoceptors And Their Interaction With Gi Proteins.
Author: Santos, Iraídes N
Sumitame, Marie
Caceres, Viviane M
Moreira, Marilia F
Krieger, Marta H
Spadari-Bratfisch, Regina C
Abstract: In this study, we investigated whether the responses of right atria from sinoaortic denervated rats to CGP12177 (4(3-t-butylamino-2-hydroxypropoxy benzidimidazole-2 one, hydrochloride)), isoprenaline and norepinephrine desensitized in parallel and whether CGP12177 interacted with distinct conformations of beta-adrenoceptors. Right atria from rats 48 h after sinoaortic denervation were subsensitive to isoprenaline, norepinephrine and CGP12177. One week after sinoaortic denervation, the sensitivity to CGP12177 had recovered whereas the responses to isoprenaline and norepinephrine were still subsensitive, suggesting that the binding sites for these molecules showed independent behavior. In atria from 48 h sinoaortic-denervated rats, propranolol or 3 microM CGP20712A (2-hydroxy-5(2-((2-hydroxy-3-(4-((methyl-4-trifluormethyl)1H imidazole-2-yl)-phenoxypropyl) amino) ethoxy)-benzamide monomethane sulphonate)) blocked the responses to 10 nM-1 microM CGP12177 and steepened the curves. The concentration-response curves to CGP12177 in the presence of ICI118,551 (erythro-DL-1(-methylindan-4-yloxy)-3-isopropylamino-butan-2-ol) were biphasic, suggesting that CGP12177 interacted with at least two conformations of beta-adrenoceptors that showed negative cooperativism, one acting through beta(2)-adrenoceptor-Gi and the other via beta(1)-adrenoceptor-Gs. This hypothesis was confirmed in right atria from sinoaortic-denervated rats treated with pertussis toxin.
Subject: Adrenergic Alpha-agonists
Adrenergic Beta-agonists
Adrenergic Beta-antagonists
Animals
Dose-response Relationship, Drug
Gtp-binding Protein Alpha Subunits, Gi-go
Heart
Heart Atria
Imidazoles
In Vitro Techniques
Isoproterenol
Male
Myocardial Contraction
Norepinephrine
Pertussis Toxin
Propanolamines
Protein Binding
Protein Conformation
Rats
Rats, Wistar
Receptors, Adrenergic, Beta
Receptors, Adrenergic, Beta-1
Rights: fechado
Identifier DOI: 10.1016/j.ejphar.2005.03.008
Address: http://www.ncbi.nlm.nih.gov/pubmed/15878715
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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