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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDetection Of Brazilian Bovine Respiratory Syncytial Virus Strain By A Reverse Transcriptase-nested-polymerase Chain Reaction In Experimentally Infected Calves.pt_BR
dc.contributor.authorAlmeida, Renata Spt_BR
dc.contributor.authorSpilki, Fernando Rpt_BR
dc.contributor.authorRoehe, Paulo Mpt_BR
dc.contributor.authorArns, Clarice Wpt_BR
unicamp.authorRenata S Almeida, Laboratório de Virologia Animal, Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, CEP 13081-970 Campinas - SP, Brazil.pt_BR
unicamp.author.externalFernando R Spilki,pt
unicamp.author.externalPaulo M Roehe,pt
unicamp.author.externalClarice W Arns,pt
dc.subjectAnimalspt_BR
dc.subjectBrazilpt_BR
dc.subjectCattlept_BR
dc.subjectCattle Diseasespt_BR
dc.subjectChick Embryopt_BR
dc.subjectLungpt_BR
dc.subjectRna, Viralpt_BR
dc.subjectRespiratory Syncytial Virus Infectionspt_BR
dc.subjectRespiratory Syncytial Virus, Bovinept_BR
dc.subjectRespiratory Tract Diseasespt_BR
dc.subjectReverse Transcriptase Polymerase Chain Reactionpt_BR
dc.subjectTracheapt_BR
dc.subjectViral Proteinspt_BR
dc.description.abstractA reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481 bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RT-nested-PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil.en
dc.relation.ispartofVeterinary Microbiologypt_BR
dc.relation.ispartofabbreviationVet. Microbiol.pt_BR
dc.date.issued2005-Janpt_BR
dc.identifier.citationVeterinary Microbiology. v. 105, n. 2, p. 131-5, 2005-Jan.pt_BR
dc.language.isoengpt_BR
dc.description.volume105pt_BR
dc.description.firstpage131-5pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0378-1135pt_BR
dc.identifier.doi10.1016/j.vetmic.2004.11.004pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/15627524pt_BR
dc.date.available2015-11-27T13:02:00Z-
dc.date.accessioned2015-11-27T13:02:00Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:02:00Z (GMT). No. of bitstreams: 1 pmed_15627524.pdf: 186018 bytes, checksum: 4a162ef6c7596cb73b3c404bc5b9df15 (MD5) Previous issue date: 2005en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196216-
dc.identifier.idPubmed15627524pt_BR
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