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Type: Artigo de periódico
Title: Characterization Of The Insulinotropic Action Of A Phospholipase A2 Isolated From Crotalus Durissus Collilineatus Rattlesnake Venom On Rat Pancreatic Islets.
Author: Nogueira, Tatiane C A
Ferreira, Fabiano
Toyama, Marcos H
Stoppiglia, Luiz Fabrizio
Marangoni, Sergio
Boschero, Antonio C
Carneiro, Everardo M
Abstract: The ability of PLA2 and crotapotin, isolated from Crotalus durissus collilineatus rattlesnake venom, to stimulate insulin secretion from isolated rat islets was examined. PLA2 and crotapotin stimulated insulin secretion at 2.8 mmol/L glucose, whereas at a high glucose concentrations (16.7 mmol/L) only PLA2 stimulated secretion. Nifedipine (10 micromol/L) did not alter the ability of PLA2 to increase insulin secretion stimulated by a depolarizing concentration of K+ (30 mmol/L). PLA2 did not affect 14CO2 production but significantly increased the efflux of arachidonic acid from isolated islets. These results indicate that PLA2-stimulated secretion is not dependent on an additional influx of Ca2+ through L-type Ca(2+)-channels but rather is associated with arachidonic acid formation in pancreatic islets.
Subject: Animals
Arachidonic Acid
Crotalid Venoms
In Vitro Techniques
Islets Of Langerhans
Phospholipases A
Phospholipases A2
Rats, Wistar
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 45, n. 2, p. 243-8, 2005-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2004.10.017
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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