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Type: Artigo de periódico
Title: Expression Of Hypoxia-inducible Factor-1alpha In The Cutaneous Lesions Of Balb/c Mice Infected With Leishmania Amazonensis.
Author: Arrais-Silva, Wagner W
Paffaro, Valdemar A
Yamada, Aureo T
Giorgio, Selma
Abstract: The hypoxia-inducible factor-1alpha (HIF-1alpha) is expressed in response to hypoxia and has been recently demonstrated in a variety of cells such as tumor cells and tumor-associated macrophages. Several characteristics of leishmanial lesions in humans and in animal models, such as microcirculation impairment, metabolic demand for leukocyte infiltration into infected tissue, parasite proliferation, and secondary bacterial infection, are strong indications of a hypoxic microenvironment in the lesions. We evaluated HIF-1alpha expression in the cutaneous lesions of BALB/c mice during Leishmania amazonensis infection. Immunohistochemical analyses of the lesions demonstrated, only in the later stages of infection when the lesion size is maximal and parasite burden is enormous and massive numbers of recruited macrophages and ulcers are observed, positive HIF-1alpha-infected cells throughout the lesions. HIF-1alpha is expressed mainly in the cytoplasm and around parasites inside the parasitophorous vacuoles of macrophages. This is the first evidence that macrophages in the microenvironment of lesions caused by a parasite produce a hypoxia-inducible factor.
Subject: Animals
Dna-binding Proteins
Disease Models, Animal
Hypoxia-inducible Factor 1
Hypoxia-inducible Factor 1, Alpha Subunit
Immunoenzyme Techniques
Leishmania Braziliensis
Leishmaniasis, Cutaneous
Mice, Inbred Balb C
Nuclear Proteins
Transcription Factors
Citation: Experimental And Molecular Pathology. v. 78, n. 1, p. 49-54, 2005-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.yexmp.2004.09.002
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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