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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleFast Screening Of Low Molecular Weight Compounds By Thin-layer Chromatography And On-spot" Maldi-tof Mass Spectrometry."pt_BR
dc.contributor.authorSantos, Leonardo Spt_BR
dc.contributor.authorHaddad, Renatopt_BR
dc.contributor.authorHöehr, Nelci Fpt_BR
dc.contributor.authorPilli, Ronaldo Apt_BR
dc.contributor.authorEberlin, Marcos Npt_BR
unicamp.authorLeonardo S Santos, Thomson Mass Spectrometry Laboratory, Institute of Chemistry, and Faculty of Medical Science, State University of Campinas, UNICAMP, Campinas, SP Brazil.pt_BR
unicamp.author.externalRenato Haddad,pt
unicamp.author.externalNelci F Höehr,pt
unicamp.author.externalRonaldo A Pilli,pt
unicamp.author.externalMarcos N Eberlin,pt
dc.subjectAlkaloidspt_BR
dc.subjectAnestheticspt_BR
dc.subjectAnti-bacterial Agentspt_BR
dc.subjectBupivacainept_BR
dc.subjectChromatography, Thin Layerpt_BR
dc.subjectIndole Alkaloidspt_BR
dc.subjectIndolespt_BR
dc.subjectMepivacainept_BR
dc.subjectMolecular Weightpt_BR
dc.subjectProtonspt_BR
dc.subjectSpectrometry, Mass, Matrix-assisted Laser Desorption-ionizationpt_BR
dc.subjectTetracyclinept_BR
dc.subjectTime Factorspt_BR
dc.description.abstractFast screening of low-MW compounds is performed by thin-layer chromatography (TLC) followed by direct on-spot matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification with nearly matrix-free mass spectra using an UV-absorbing ionic liquid matrix. Owing to minimal background ions from the proton donor triethylamine/alpha-cyano-4-hydroxycinnamic acid ionic liquid matrix, three arborescidine alkaloids, the anesthesics levobupivacaine and mepivacaine, and the antibiotic tetracycline were readily characterized most frequently by the MS detection of their protonated molecules. The technique is fast and sensitive, requires little sample preparation and manipulation, and is therefore suitable for fast screening with TLC separation and MS identification of low-MW compounds, with potential applications in areas such as phytochemistry, synthetic chemistry, and product manufacturing quality monitoring.en
dc.relation.ispartofAnalytical Chemistrypt_BR
dc.relation.ispartofabbreviationAnal. Chem.pt_BR
dc.date.issued2004-Aprpt_BR
dc.identifier.citationAnalytical Chemistry. v. 76, n. 7, p. 2144-7, 2004-Apr.pt_BR
dc.language.isoengpt_BR
dc.description.volume76pt_BR
dc.description.firstpage2144-7pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0003-2700pt_BR
dc.identifier.doi10.1021/ac035387dpt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/15053682pt_BR
dc.date.available2015-11-27T12:58:50Z-
dc.date.accessioned2015-11-27T12:58:50Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:58:50Z (GMT). No. of bitstreams: 1 pmed_15053682.pdf: 121810 bytes, checksum: 2a8be4fde7cdb69922fcda2b52b6aad8 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196180-
dc.identifier.idPubmed15053682pt_BR
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