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Type: Artigo de periódico
Title: Fast Screening Of Low Molecular Weight Compounds By Thin-layer Chromatography And On-spot" Maldi-tof Mass Spectrometry."
Author: Santos, Leonardo S
Haddad, Renato
Höehr, Nelci F
Pilli, Ronaldo A
Eberlin, Marcos N
Abstract: Fast screening of low-MW compounds is performed by thin-layer chromatography (TLC) followed by direct on-spot matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification with nearly matrix-free mass spectra using an UV-absorbing ionic liquid matrix. Owing to minimal background ions from the proton donor triethylamine/alpha-cyano-4-hydroxycinnamic acid ionic liquid matrix, three arborescidine alkaloids, the anesthesics levobupivacaine and mepivacaine, and the antibiotic tetracycline were readily characterized most frequently by the MS detection of their protonated molecules. The technique is fast and sensitive, requires little sample preparation and manipulation, and is therefore suitable for fast screening with TLC separation and MS identification of low-MW compounds, with potential applications in areas such as phytochemistry, synthetic chemistry, and product manufacturing quality monitoring.
Subject: Alkaloids
Anti-bacterial Agents
Chromatography, Thin Layer
Indole Alkaloids
Molecular Weight
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Time Factors
Citation: Analytical Chemistry. v. 76, n. 7, p. 2144-7, 2004-Apr.
Rights: fechado
Identifier DOI: 10.1021/ac035387d
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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