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Type: Artigo de periódico
Title: Rapid Method For Evaluating Reversed-phase High-performance Liquid Chromatography Column Stability.
Author: Fonseca, Dania A
Gutiérrez, Humberto R
Collins, Kenneth E
Collins, Carol H
Abstract: A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60 degrees C. Mobile phase (MeOH-0.1 mol l(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process.
Subject: Chromatography, High Pressure Liquid
Hydrogen-ion Concentration
Citation: Journal Of Chromatography. A. v. 1030, n. 1-2, p. 149-55, 2004-Mar.
Rights: fechado
Identifier DOI: 
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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