Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Analysis Of Cellular Morphology, Adhesion, And Proliferation On Uncoated And Differently Coated Pvc Tubes Used In Extracorporeal Circulation (ecc).
Author: Moreira, Patrícia da Luz
Marreco, Paula Rulf
Moraes, Angela Maria
Wada, Maria Lúcia Furlan
Genari, Selma Candelária
Abstract: The biggest challenge to improve extracorporeal circulation (ECC) circuits lays on avoiding platelet adhesion to their surfaces, because this contributes to thrombus formation, resulting in the activation of blood coagulation. One approach to minimize this effect is to improve the biocompatibility of ECC circuits by modifying their surfaces. This can be achieved by coating them with heparin or phospholipids. The present study investigated the adhesion and morphology characteristics of fibroblastic and blood cells cultured on uncoated poly (vinyl) chloride PVC tubes as well as on heparin, phosphatidylcholine (DMPC), and phosphatidylethanolamine (DMPE) -coated tubing. The results showed the importance of uniform coating regardless of the substance used, because the coatings cover the grooves on PVC surfaces, which favor cell adhesion. The comparison among the three different coatings showed the best biocompatibility results for the PVC tubes coated with heparin, followed by the coating with DMPE and with DMPC. For all coated tubes, cells did not spread on the PVC surfaces and, consequently, did not adhere to their surfaces, increasing the overall biocompatibility of PVC tubes. However, possible DMPE's alkylation, caused by sterilization, resulted in increased material hydrophobicity, which explains the decrease in fibroblastic adhesion. Furthermore, sterilization of DMPC-PVC improves its hydrophilic character, also decreasing adhesion. Based on these results, coating PVC with the phospholipids DMPC and DMPE seems to be a promising technique to improve the biocompatibility of PVC tubes, and is worthy of further investigation.
Subject: Animals
Blood Cells
Cell Adhesion
Cell Division
Cell Line
Cercopithecus Aethiops
Extracorporeal Circulation
Microscopy, Electron, Scanning
Mitotic Index
Polyvinyl Chloride
Vero Cells
Citation: Journal Of Biomedical Materials Research. Part B, Applied Biomaterials. v. 69, n. 1, p. 38-45, 2004-Apr.
Rights: fechado
Identifier DOI: 10.1002/jbm.b.20028
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_15015208.pdf346.23 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.