Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196045
Type: Artigo de periódico
Title: Extractive Cultivation Of Xylanase By Penicillium Janthinellum In A Poly(ethylene Glycol)/cashew-nut Tree Gum Aqueous Two-phase System.
Author: Oliveira, Luciana A
Barros Neto, Benício
Porto, Ana L F
Tambourgi, Elias B
Abstract: Cultivation of the fungus Penicillium janthinellum for xylanase production was studied in a poly(ethylene glycol)/cashew-nut tree gum aqueous two-phase system, using a two-level fractional factorial design. The parameters studied were initial pH, cultivation time, type of agro-industrial residue (oat husk or corn cob), agitation, temperature, and phase-forming polymers. The xylanase produced during fermentation partitioned into the top phase. The agitation and temperature (negative), cultivation time and initial pH (positive) effects proved statistically significant for xylanase production. The highest percentage yield of the xylanase in the top and its production in the top phase, about 97% and 160.7 U/mL, were obtained in cultures of 120 h, 40 rpm, 25 degrees C, and pH 5.0.
Subject: Anacardium
Bioreactors
Cell Culture Techniques
Chemical Fractionation
Endo-1,4-beta Xylanases
Enzyme Activation
Enzyme Stability
Hydrogen-ion Concentration
Penicillium
Plant Extracts
Polyethylene Glycols
Temperature
Rights: fechado
Identifier DOI: 10.1021/bp049746h
Address: http://www.ncbi.nlm.nih.gov/pubmed/15575727
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_15575727.pdf136.13 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.