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Type: Artigo de periódico
Title: Rarity Of Dna Sequence Alterations In The Promoter Region Of The Human Androgen Receptor Gene.
Author: Cabral, D F
Santos, A
Ribeiro, M L
Mesquita, J C
Carvalho-Salles, A B
Hackel, C
Abstract: The human androgen receptor (AR) gene promoter lies in a GC-rich region containing two principal sites of transcription initiation and a putative Sp1 protein-binding site, without typical TATA and CAAT boxes. It has been suggested that mutations within the 5'untranslated region (5'UTR) may contribute to the development of prostate cancer by changing the rates of gene transcription and/or translation. In order to investigate this question, the aim of the present study was to search for the presence of mutations or polymorphisms at the AR-5'UTR in 92 prostate cancer patients, where histological diagnosis of adenocarcinoma was established in specimens obtained from transurethral resection or after prostatectomy. The AR-5'UTR was amplified by PCR from genomic DNA samples of the patients and of 100 healthy male blood donors, included as controls. Conformation-sensitive gel electrophoresis was used for DNA sequence alteration screening. Only one band shift was detected in one individual from the blood donor group. Sequencing revealed a new single nucleotide deletion (T) in the most conserved portion of the promoter region at position +36 downstream from the transcription initiation site I. Although the effect of this specific mutation remains unknown, its rarity reveals the high degree of sequence conservation of the human androgen promoter region. Moreover, the absence of detectable variation within the critical 5'UTR in prostate cancer patients indicates a low probability of its involvement in prostate cancer etiology.
Subject: 5' Untranslated Regions
Case-control Studies
Genetic Markers
Middle Aged
Polymorphism, Genetic
Promoter Regions, Genetic
Prostatic Neoplasms
Receptors, Androgen
Sequence Analysis, Dna
Rights: aberto
Identifier DOI: /S0100-879X2004001200004
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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