Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196030
Type: Artigo de periódico
Title: Cytotoxicity Of The Phytosterol Diosgenin And Its Derivatives In Rat Cultured Hepatocytes And V79 Fibroblasts.
Author: Melo, Patricia Silva
De Azevedo, Mariangela Burgos Martins
Zullo, Marco Antônio Teixeira
Fabrin-Neto, João Batista
Haun, Marcela
Abstract: In this work, the cytotoxic effects of some spirostane derivatives were examined in cultured hepatocytes and V79 fibroblasts using different viability assays. The derivatives were obtained by modifying the A and B rings of diosgenin. Diosgenin and its derivatives were more toxic in V79 fibroblasts (IC50 40-300 microM) than in hepatocytes (IC50 280-1000 microM). Inhibition of cytochrome P450IIIA in cultured hepatocytes by incubation with 1 mM cimetidine did not alter the toxicity of these compounds in these cells. These observations suggest that other pathways of detoxification may be involved in hepatocytes. In conclusion, the compounds studied merit investigation for their potential pharmacological and industrial applicability.
Subject: Animals
Cell Count
Cell Line
Cell Survival
Cimetidine
Cricetinae
Cricetulus
Dna
Diosgenin
Dose-response Relationship, Drug
Drug Combinations
Fibroblasts
Hepatocytes
Neutral Red
Rats
Tetrazolium Salts
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/15553174
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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