Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.typeArtigo de periódicopt_BR
dc.titleInteraction Of Non-ionic Surfactants With Hepatic Cyp In Prochilodus Scrofa.pt_BR
dc.contributor.authorda Silva, Maria E Fpt_BR
dc.contributor.authorMeirelles, Nilce Cpt_BR
unicamp.authorMaria E F da Silva, Laboratory of Biomembranes, Department of Biochemistry, Institute of Biology, State University of Campinas, CP6109, ZIP CODE 13083-970, Cidade Universitária Zeferino Vaz, Barão Geraldo, Campinas, SP, Brazil. feracin@unicamp.brpt_BR C Meirelles,pt
dc.subjectBiological Markerspt_BR
dc.subjectCytochrome P-450 Cyp1a1pt_BR
dc.subjectCytochrome P-450 Enzyme Systempt_BR
dc.subjectReproducibility Of Resultspt_BR
dc.subjectSurface-active Agentspt_BR
dc.description.abstractCytochromes P450 (CYP) constitute a superfamily of hemeproteins that play a vital role in the metabolism of a wide variety of endogenous and xenobiotic compounds. Xenobiotic metabolism and the role of CYP are of particular interest in studies regarding the prevention of the damage caused by chemical pollutants. We investigated, in this study, the interaction of Triton X-100 and Tween 80 with CYP and antioxidant defenses in Curimbata, a Brazilian fish. Aiming to clarify the effects of non-ionic surfactants in the monooxigenase system of fish through in vitro study, the effects of Triton X-100 and Tween 80 were analyzed using monooxygenases and antioxidant system as experimental model. Total CYP and EROD were strongly inhibited by Triton X-100 and Tween 80 in a concentration-dependent way; the content of CYP was reduced until zero while EROD activity was completely inhibited in the presence of Triton X-100 and more than 40% inhibited in the presence of Tween 80. Each surfactant causes a different effect on each antioxidant enzyme. No effect was detected in SOD activity in the presence of even Triton X-100 or Tween 80. Triton X-100 increase catalase activity, while Tween 80 decreases this enzyme activity. The molecular structure of the surfactants causes the alteration of this system, since they are able to interact with the microsomal protein, especially with monooxigenase's components, altering their conformation and, consequently destroying their function. Our results suggest that surfactants can interact with components of the microsomal system leading to inhibition of CYP. Therefore, CYP activity, which has been used as a biomarker of xenobiotic exposure, should be used as a marker in association with other enzymes.en
dc.relation.ispartofToxicology In Vitro : An International Journal Published In Association With Bibrapt_BR
dc.relation.ispartofabbreviationToxicol In Vitropt_BR
dc.identifier.citationToxicology In Vitro : An International Journal Published In Association With Bibra. v. 18, n. 6, p. 859-67, 2004-Dec.pt_BR
dc.description.provenanceMade available in DSpace on 2015-11-27T12:58:29Z (GMT). No. of bitstreams: 1 pmed_15465653.pdf: 378347 bytes, checksum: 4b50faa6bf51e96247c697a3fc7ddcca (MD5) Previous issue date: 2004en
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_15465653.pdf369.48 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.